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Literature summary extracted from

  • Kawasaki, H.; Nakamura, N.; Ohmori, M.; Amari, K.; Sakai, T.
    Screening for bacteria producing sucrose phosphorylase and characterization of the enzymes (1996), Biosci. Biotechnol. Biochem., 60, 319-321.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 12
-
 D-fructose
-
 Leuconostoc mesenteroides
2.4.1.7 16
-
 alpha-D-glucose 1-phosphate
-
 Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 54000
-
 SDS-PAGE, ATCC 12291 Leuconostoc mesenteroides
2.4.1.7 58000
-
 SDS-PAGE, AKU 1102 Leuconostoc mesenteroides
2.4.1.7 58000
-
 gel filtration, ATCC 12291 Leuconostoc mesenteroides
2.4.1.7 60000
-
 gel filtration, AKU 1102 Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides ping-pong mechanism alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides AKU 1102 ping-pong mechanism alpha-D-glucose 1-phosphate + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides
-
 AKU 1102
-
2.4.1.7 Leuconostoc mesenteroides
-
 ATCC 12291
-
2.4.1.7 Leuconostoc mesenteroides AKU 1102
-
 AKU 1102
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7
-
 Leuconostoc mesenteroides

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.7 sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate ping-pong mechanism Leuconostoc mesenteroides
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 37.4
-
 purified enzyme from AKU 1102 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-phosphate + D-xylulose
-
 Leuconostoc mesenteroides alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + D-xylulose
-
 Leuconostoc mesenteroides AKU 1102 alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + L-arabinose
-
 Leuconostoc mesenteroides ?
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + L-arabinose
-
 Leuconostoc mesenteroides AKU 1102 ?
-
 r
2.4.1.7 alpha-D-glucose-1-phosphate + L-sorbose
-
 Leuconostoc mesenteroides alpha-D-glucosyl-alpha-L-sorbose + phosphate
-
 r
2.4.1.7 alpha-D-glucose-1-phosphate + L-sorbose
-
 Leuconostoc mesenteroides AKU 1102 alpha-D-glucosyl-alpha-L-sorbose + phosphate
-
 r
2.4.1.7 sucrose + phosphate ping-pong mechanism Leuconostoc mesenteroides alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate ping-pong mechanism Leuconostoc mesenteroides alpha-D-glucose 1-phosphate + D-fructose
-
 r
2.4.1.7 sucrose + phosphate ping-pong mechanism Leuconostoc mesenteroides AKU 1102 alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate ping-pong mechanism Leuconostoc mesenteroides AKU 1102 alpha-D-glucose 1-phosphate + D-fructose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.7 monomer gel filtration, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 37 40 reverse reaction Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.7 40
-
 rapid loss of activity above 40°C Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5 6 reverse reaction Leuconostoc mesenteroides
2.4.1.7 7 7.5 forward reaction Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.7 5 8.5 stable at 30°C Leuconostoc mesenteroides