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Literature summary extracted from

  • Doudoroff, M.
    Disaccharide phosphorylases (1961), The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. ), 5, 229-236.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.7 unstable in cell extract Shewanella putrefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.7 D-glucose strong inhibition Leuconostoc mesenteroides
2.4.1.7 D-glucose strong inhibition Pelomonas saccharophila
2.4.1.7 D-glucose strong inhibition Shewanella putrefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides highly specific for alpha-D-glycosyl configuration alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate Pelomonas saccharophila highly specific for alpha-D-glycosyl configuration alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate Shewanella putrefaciens highly specific for alpha-D-glycosyl configuration alpha-D-glucose 1-phosphate + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides
-
-
-
2.4.1.7 Pelomonas saccharophila
-
-
-
2.4.1.7 Shewanella putrefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7 partial Leuconostoc mesenteroides
2.4.1.7 partial Pelomonas saccharophila
2.4.1.7 partial Shewanella putrefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-phosphate + D-xylulose
-
 Leuconostoc mesenteroides alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + D-xylulose
-
 Pelomonas saccharophila alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + H2O
-
 Leuconostoc mesenteroides alpha-D-glucose + phosphate
-
 ir
2.4.1.7 alpha-D-glucose 1-phosphate + H2O
-
 Pelomonas saccharophila alpha-D-glucose + phosphate
-
 ir
2.4.1.7 alpha-D-glucose 1-phosphate + H2O
-
 Shewanella putrefaciens alpha-D-glucose + phosphate
-
 ir
2.4.1.7 alpha-D-glucose-1-phosphate + L-sorbose
-
 Leuconostoc mesenteroides alpha-D-glucosyl-alpha-L-sorbose + phosphate
-
 r
2.4.1.7 alpha-D-glucose-1-phosphate + L-sorbose
-
 Pelomonas saccharophila alpha-D-glucosyl-alpha-L-sorbose + phosphate
-
 r
2.4.1.7 glucose-1-phosphate + arsenate
-
 Leuconostoc mesenteroides glucose-1-arsenate + phosphate glucose-1-arsenate is further hydrolyzed to form glucose and arsenate ir
2.4.1.7 glucose-1-phosphate + arsenate
-
 Pelomonas saccharophila glucose-1-arsenate + phosphate glucose-1-arsenate is further hydrolyzed to form glucose and arsenate ir
2.4.1.7 glycosyl-glucose + arsenate
-
 Leuconostoc mesenteroides glucose-1-arsenate + glucose glucose-1-arsenate is further hydrolyzed to form glucose and arsenate ?
2.4.1.7 additional information D-fructose can not be replaced with L-sorbose or D-xylulose in reverse reaction Shewanella putrefaciens ?
-
 ?
2.4.1.7 additional information sucrose and alpha-glucose-1-phosphate are hydrolyzed in absence of phosphate and arsenate at very low rate Leuconostoc mesenteroides ?
-
 ?
2.4.1.7 additional information sucrose and alpha-glucose-1-phosphate are hydrolyzed in absence of phosphate and arsenate at very low rate Pelomonas saccharophila ?
-
 ?
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Leuconostoc mesenteroides alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Leuconostoc mesenteroides alpha-D-glucose 1-phosphate + D-fructose
-
 r
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Pelomonas saccharophila alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Pelomonas saccharophila alpha-D-glucose 1-phosphate + D-fructose
-
 r
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Shewanella putrefaciens alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate highly specific for alpha-D-glycosyl configuration Shewanella putrefaciens alpha-D-glucose 1-phosphate + D-fructose
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.8
-
-
 Leuconostoc mesenteroides
2.4.1.7 6.8
-
-
 Pelomonas saccharophila
2.4.1.7 6.8
-
-
 Shewanella putrefaciens