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Literature summary extracted from

  • Yamagata, S.; Paszewski, A.; Lewandowska, I.
    Purification and properties of O-acetyl-L-homoserine O-sulfhydrylase from Aspergillus nidulans (1990), J. Gen. Appl. Microbiol., 36, 137-141.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.49 existence of glycerol in the purification buffer keeps the enzyme very stable, sensitive to salts such as (NH4)2SO4 and NaCl in absence of glycerol Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 DL-C-propagylglycine only 25% inhibition Aspergillus nidulans
2.5.1.49 L-methionine only 20% inhibition Aspergillus nidulans
2.5.1.49 additional information no sulfhydryl reagents inhibits the enzyme, S-adenosylmethionine is no inhibitor Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 43000
-
 6 * 43000, SDS-PAGE Aspergillus nidulans
2.5.1.49 250000 260000 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Aspergillus nidulans
-
 L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S Aspergillus nidulans sG8
-
 L-homocysteine + acetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Aspergillus nidulans
-
 and strains pabaA2, YA1
-
2.5.1.49 Aspergillus nidulans sG8
-
 and strains pabaA2, YA1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.49 mycelium
-
 Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 8.96
-
-
 Aspergillus nidulans

Storage Stability

EC Number Storage Stability Organism
2.5.1.49 -20°C, stable for at least 6 months Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Aspergillus nidulans L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Aspergillus nidulans sG8 L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-serine + H2S
-
 Aspergillus nidulans L-cysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-serine + H2S
-
 Aspergillus nidulans sG8 L-cysteine + acetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.49 hexamer 6 * 43000, SDS-PAGE Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.49 60
-
 stable at Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 7.5
-
 O-acetyl-L-serine as substrate Aspergillus nidulans
2.5.1.49 8
-
 O-acetyl-L-homoserine as substrate Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Aspergillus nidulans