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Literature summary extracted from

  • Yamagata, S.
    O-Acetylhomoserine sulfhydrylase of the fission yeast Schizosaccharomyces pombe: Partial purification, characterization, and its probable role in homocysteine biosynthesis (1984), J. Biochem., 96, 1511-1523.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Schizosaccharomyces pombe

General Stability

EC Number General Stability Organism
2.5.1.49 very unstable, can be stabilized by addition of 25% sucrose or glycerol Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 DL-Ethionine
-
 Schizosaccharomyces pombe
2.5.1.49 Hydroxylamine hydrochloride
-
 Schizosaccharomyces pombe
2.5.1.49 KCN
-
 Schizosaccharomyces pombe
2.5.1.49 L-methionine competitive inhibition Schizosaccharomyces pombe
2.5.1.49 L-Penicillamine
-
 Schizosaccharomyces pombe
2.5.1.49 additional information p-chloromercuribenzoic acid, 5,5'-dithio-bis(2-nitrobenzoic acid) and monoiodoacetic acid have no inhibitory effect Schizosaccharomyces pombe
2.5.1.49 O-acetyl-L-serine
-
 Schizosaccharomyces pombe
2.5.1.49 O-phospho-L-serine
-
 Schizosaccharomyces pombe
2.5.1.49 phenylhydrazine
-
 Schizosaccharomyces pombe
2.5.1.49 S-adenosyl-L-homocysteine
-
 Schizosaccharomyces pombe
2.5.1.49 S-adenosyl-L-methionine
-
 Schizosaccharomyces pombe
2.5.1.49 Semicarbazide
-
 Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 0.053
-
 H2S
-
 Schizosaccharomyces pombe
2.5.1.49 10.4
-
 L-homoserine
-
 Schizosaccharomyces pombe
2.5.1.49 11.1
-
 O-succinyl-L-homoserine
-
 Schizosaccharomyces pombe
2.5.1.49 12.5
-
 O-acetyl-L-homoserine
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 170000
-
 sucrose density gradient ultracentrifugation Schizosaccharomyces pombe
2.5.1.49 186000
-
 gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Schizosaccharomyces pombe
-
 L-homocysteine + acetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Schizosaccharomyces pombe
-
 fission yeast, IFO-0363
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Schizosaccharomyces pombe

Storage Stability

EC Number Storage Stability Organism
2.5.1.49 -20°C, can be stored without loss of catalytic activity Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 L-homoserine + H2S
-
 Schizosaccharomyces pombe homocysteine + ?
-
 ?
2.5.1.49 additional information does not synthesize cystathionine from O-acetyl-L-homoserine and L-cysteine, cannot utilize cysteine as co-substrate in place of H2S, O-acetyl-L-serine, O-acetyl-L-tyrosine, O-acetyl-L-threonine, O-phospho-DL-threonine, O-phospho-L-serine, L-serine-O-sulfate and O-acetyl-L-hydroxyproline are no substrates Schizosaccharomyces pombe ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Schizosaccharomyces pombe L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-serine + H2S
-
 Schizosaccharomyces pombe L-cysteine + acetic acid
-
 ?
2.5.1.49 O-succinyl-L-homoserine + H2S
-
 Schizosaccharomyces pombe homocysteine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.49 Homocysteine synthase
-
 Schizosaccharomyces pombe
2.5.1.49 OAH sulfhydrylase
-
 Schizosaccharomyces pombe

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.49 30
-
 more stable at 30°C than at 0°C Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 8
-
-
 Schizosaccharomyces pombe

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.49 7
-
 optimal pH for stability Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Schizosaccharomyces pombe

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.49 2.6
-
 L-methionine
-
 Schizosaccharomyces pombe