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Literature summary extracted from

  • Kerr, D.S.
    O-Acetylhomoserine sulfhydrylase (Neurospora) (1971), Methods Enzymol., 17, 446-450.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.49 enzyme appears to be quite stable Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 additional information no end product inhibition by methionine or S-adenosylmethionine observed Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 0.7
-
 H2S
-
 Neurospora crassa
2.5.1.49 0.8
-
 methyl-mercaptan
-
 Neurospora crassa
2.5.1.49 7
-
 O-acetyl-L-homoserine
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Neurospora crassa
-
 L-homocysteine + acetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 4
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.5.1.49 -15°C, 80% of the activity remains after storage for 6 months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 additional information L-homoserine, O-succinyl-DL-homoserine, O-phosphoryl-L-homoserine, L-serine, O-acetyl-L-serine, O-phosphoryl-L-serine react at less than 1% the rate with O-acetyl-L-homoserine Neurospora crassa ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Neurospora crassa L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methyl mercaptan
-
 Neurospora crassa L-methionine + acetic acid
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate only required after enzyme is resolved by dialysis against cysteine Neurospora crassa