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Literature summary extracted from

  • Watson, D.; Jourdian, G.W.; Roseman, S.
    N-Acylneuraminic (sialic) acid 9-phosphate synthetase (1966), Methods Enzymol., 8, 201-205.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.57 purified enzyme is unstable Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.57 EDTA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.57 0.69
-
 N-acetyl-D-mannosamine 6-phosphate
-
 Sus scrofa
2.5.1.57 1.6
-
 N-glycolyl-D-mannosamine 6-phosphate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.57 Co2+
-
 Sus scrofa
2.5.1.57 Mg2+ maximal enzyme activity in presence of 0.02 M Sus scrofa
2.5.1.57 Mn2+
-
 Sus scrofa
2.5.1.57 additional information Ca2+ or Cu2+ does not substitute for Mg2+ Sus scrofa
2.5.1.57 Ni2+
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Sus scrofa
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.57 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.57
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.57 submaxillary gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.57 0.83
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.5.1.57 -20°C, crude extract stable for at least 1 month Sus scrofa
2.5.1.57 0°C, loses approximately 25% of the activity during a 24-h period Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-glycolyl-neuraminate 9-phosphate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.57 7.8
-
-
 Sus scrofa