Any feedback?
Literature summary extracted from
- Partial purification and characterization of UDPG:t-cinnamate glucosyltransferase in the root of sweet potato, Ipomoea batatas Lam (1984), J. Biochem., 95, 205-212.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.177 | freezing inactivates, sorbitol stabilizes | Ipomoea batatas |
| 2.4.1.177 | PMSF and 2-mercaptoethanol stabilize during purification | Ipomoea batatas |
| 2.4.1.177 | sorbitol, 10%, stabilizes during storage | Ipomoea batatas |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.177 | HgCl2 | strong | Ipomoea batatas |  |
| 2.4.1.177 | additional information | no inhibition by MgCl2, CaCl2, MnCl2, chlorogenic acid (1 mM each) | Ipomoea batatas | |
| 2.4.1.177 | p-chloromercuribenzoate | - |
Ipomoea batatas | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.177 | 0.1 | - |
UDP-glucose | - |
Ipomoea batatas | |
| 2.4.1.177 | 0.2 | - |
trans-cinnamate | - |
Ipomoea batatas | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.177 | soluble | - |
Ipomoea batatas | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.177 | additional information | no activation by MgCl2, CaCl2 or MnCl2, 1 mM each | Ipomoea batatas |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.177 | 45000 | - |
gel filtration | Ipomoea batatas |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.177 | UDP-alpha-D-glucose + cinnamate | Ipomoea batatas | involved in biosynthesis of chlorogenic acid in root of Ipomoea batatas | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.177 | Ipomoea batatas | - |
sweet potato, Lam cv. Norin 1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.177 | partial | Ipomoea batatas |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.177 | root | - |
Ipomoea batatas | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.177 | 70.1 | - |
- |
Ipomoea batatas |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.177 | -20°C, 10% sorbitol, about 10% loss of activity within 30 days | Ipomoea batatas |
| 2.4.1.177 | sorbitol, 10%, stabilizes during storage | Ipomoea batatas |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.177 | UDP-alpha-D-glucose + caffeate | glucosylation at 15% the rate of cinnamate | Ipomoea batatas | UDP + 1-O-caffeoyl-beta-D-glucose | - |
? | |
| 2.4.1.177 | UDP-alpha-D-glucose + cinnamate | best substrate | Ipomoea batatas | UDP + 1-O-cinnamoyl-beta-D-glucose | trans-isomer | ? | |
| 2.4.1.177 | UDP-alpha-D-glucose + cinnamate | trans-cinnamate | Ipomoea batatas | UDP + 1-O-cinnamoyl-beta-D-glucose | trans-isomer | ? | |
| 2.4.1.177 | UDP-alpha-D-glucose + cinnamate | involved in biosynthesis of chlorogenic acid in root of Ipomoea batatas | Ipomoea batatas | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
| 2.4.1.177 | UDP-alpha-D-glucose + ferulate | glucosylation at 27% the rate of cinnamate | Ipomoea batatas | UDP + 1-O-feruloyl-beta-D-glucose | - |
? | |
| 2.4.1.177 | UDP-glucose + 4-hydroxy-3-methoxycinnamate | glucosylation at 35% the rate of cinnamate | Ipomoea batatas | UDP + 4-hydroxy-3-methoxycinnamoyl beta-D-glucoside | - |
? | |
| 2.4.1.177 | UDP-glucose + benzoate | glucosylation at 71% the rate of cinnamate | Ipomoea batatas | UDP + benzoyl-beta-D-glucoside | - |
? | |
| 2.4.1.177 | UDP-glucose + o-coumarate | glucosylation at 52% the rate of cinnamate | Ipomoea batatas | UDP + o-coumaroyl beta-D-glucose | - |
? | |
| 2.4.1.177 | UDP-glucose + p-coumarate | the glucosyl-group is introduced exclusively into the carboxyl-group | Ipomoea batatas | UDP + p-coumaroyl-D-glucose | - |
? | |
| 2.4.1.177 | UDP-glucose + p-coumarate | glucosylation at 57% the rate of cinnamate | Ipomoea batatas | UDP + p-coumaroyl-D-glucose | - |
? | |
| 2.4.1.177 | UDP-glucose + trans-cinnamate | - |
Ipomoea batatas | UDP + trans-cinnamoyl beta-D-glucoside | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.177 | 30 | - |
assay at | Ipomoea batatas |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.177 | 5.8 | - |
- |
Ipomoea batatas |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
