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Literature summary extracted from

  • Hatfield, D.; Wyngaarden, J.B.
    3-Ribosylpurines. I.Synthesis of (3-ribosyluric acid) 5phosphate and (3-ribosylxanthine) 5phosphate by a pyrimidine ribonucleotide pyrophosphorylase (1964), J. Biol. Chem., 239, 2580-2586.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.20 3-ribosylxanthine 5'-phosphate xanthine and uracil pyrophosphorylase Bos taurus
2.4.2.20 UMP xanthine and uracil pyrophosphorylase Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.20 Mg2+ required at 2 mM or higher for maximal activity Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.20 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.20 purified 5400fold, ammonium sulfate fractionation, chromatography on DEAE-cellulose, treatment with Alumina CY Gel, calcium phosphate gel adsorption and elution and purification with hydroxylapatite Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.20 erythrocyte
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.20 0.497
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.20 additional information enzyme also synthesizes a number of pyrimidine ribonucleotides from the corresponding base, e.g. uracil, orotic acid, thymine, 6-azathymine, 6-azauracil, 5-fluorouracil or 5-iodouracil and diphosphorylribose phosphate Bos taurus ?
-
 ?
2.4.2.20 uric acid + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Bos taurus 3-ribosyluric acid 5'-phosphate + diphosphate
-
 ?
2.4.2.20 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Bos taurus 3-ribosylxanthine 5'-phosphate + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.20 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.20 50
-
 pH 6.0, 40 min, 12-22% loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.20 6
-
 for uric acid reaction Bos taurus
2.4.2.20 7.6 8.6 for xanthine reaction Bos taurus
2.4.2.20 8 9.2 for orotic acid reaction Bos taurus
2.4.2.20 9.5
-
 for uracil reaction Bos taurus