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Literature summary extracted from

  • Kniep, B.; Grisebach, H.
    Biosynthesis of streptomycin. Purification and properties of a dTDP-L-dihydrostreptose:streptidine-6-phosphate dihydrostreptosyltransferase from Streptomyces griseus (1980), Eur. J. Biochem., 105, 139-144.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.27 addition of streptidine is important for stabilization of enzyme activity during purification, purified transferase is also stable in absence of streptidine Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.27 dTDP
-
Streptomyces griseus
2.4.2.27 dTTP
-
Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.27 Co2+ as effective as Mg2+ Streptomyces griseus
2.4.2.27 Mg2+ Mn2+ or Mg2+ required, maximal activity with 10 mM Mg2+ Streptomyces griseus
2.4.2.27 Mn2+ Mn2+ or Mg2+ required, maximal activity with 3 mM Mn2+ Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.27 35000
-
2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus
2.4.2.27 63000
-
gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.27 dTDP-L-dihydrostreptose + streptidine 6-phosphate Streptomyces griseus biosynthesis of streptomycin ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.27 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.27 ammonium sulfate precipitation, Sephadex G-200, chromatography on DEAE-cellulose, Ultrogel AcA44 column, DEAE-Sepharose column Streptomyces griseus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.27 additional information
-
-
Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
2.4.2.27 4°C, 50 mM Tris-HCl, pH 7.8 at 0°C, containing 5 mM mercaptoethanol, 1 mM EDTA, 2.5 mM streptidine, 10% v/v glycerol, stable for at least 4 months Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.27 dTDP-L-dihydrostreptose + streptidine 6-phosphate
-
Streptomyces griseus dTDP + O-alpha-L-dihydrostreptosyl-1,4-streptidine 6-phosphate
-
?
2.4.2.27 dTDP-L-dihydrostreptose + streptidine 6-phosphate biosynthesis of streptomycin Streptomyces griseus ?
-
?
2.4.2.27 additional information not: streptidine, 2-deoxystreptamine, 4-deoxystreptamine Streptomyces griseus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.27 dimer 2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.27
-
-
assay at Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.27 additional information
-
no distinct pH-optimum observed in Tris-HCl buffer between pH 7.3 and 9 Streptomyces griseus
2.4.2.27 8.2
-
glycine/NaOH buffer Streptomyces griseus