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Literature summary extracted from

  • Ortmann, R.; Sutter, A.; Grisebach, H.
    Purification and properties of udpapiose: 7-O-(beta-D-glucosyl)-flavone apiosyltransferase from cell suspension cultures of parsley (1972), Biochim. Biophys. Acta, 289, 293-302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.25 dithioerythritol stimulates enzyme activity up to 35%, optimum concentration: 6 mM Petroselinum crispum

General Stability

EC Number General Stability Organism
2.4.2.25 albumin, 0.1 mg, significant stabilization Petroselinum crispum
2.4.2.25 solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.25 apigenin-7-glucoside
-
Petroselinum crispum
2.4.2.25 apiin
-
Petroselinum crispum
2.4.2.25 Ca2+
-
Petroselinum crispum
2.4.2.25 Chrysoeriol-7-glucoside
-
Petroselinum crispum
2.4.2.25 Co2+
-
Petroselinum crispum
2.4.2.25 iodoacetamide at 1 mM causes an inhibition of 55%, reversed by cysteine Petroselinum crispum
2.4.2.25 Mg2+
-
Petroselinum crispum
2.4.2.25 Mn2+
-
Petroselinum crispum
2.4.2.25 additional information no effect: EDTA, NH4+, K+ Petroselinum crispum
2.4.2.25 p-chloromercuribenzoate at 1 mM inhibits the reaction completely Petroselinum crispum
2.4.2.25 UDP
-
Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.25 0.066
-
apigenin-7-glucoside
-
Petroselinum crispum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.25 soluble
-
Petroselinum crispum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.25 50000
-
gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.25 UDPapiose + 7-O-(beta-D-glucosyl)-apigenin Petroselinum crispum biosynthesis of apiin ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.25 Petroselinum crispum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.25 purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.25 cell suspension culture
-
Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.25 additional information
-
-
Petroselinum crispum

Storage Stability

EC Number Storage Stability Organism
2.4.2.25 -20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.25 additional information overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose Petroselinum crispum ?
-
?
2.4.2.25 UDPapiose + 7-O-(beta-D-glucosyl)-apigenin specific for UDPapiose as glycosyl donor Petroselinum crispum UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin i.e. apiin ?
2.4.2.25 UDPapiose + 7-O-(beta-D-glucosyl)-apigenin biosynthesis of apiin Petroselinum crispum ?
-
?
2.4.2.25 UDPapiose + biochanin A i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside Petroselinum crispum ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.25 30
-
assay at Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.25 7
-
higher apiin yield in Tris-HCl than in phosphate buffer Petroselinum crispum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.25 6 8 highest stability Petroselinum crispum