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Literature summary extracted from

  • Warnecke, D.; Erdmann, R.; Fahl, A.; Hube, B.; Muller, F.; Zank, T.; Zahringer, U.; Heinz, E.
    Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum (1999), J. Biol. Chem., 274, 13048-13059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.173 expression in Escherichia coli Candida albicans
2.4.1.173 expression in Escherichia coli Dictyostelium discoideum
2.4.1.173 expression in Escherichia coli Komagataella pastoris
2.4.1.173 expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.173 additional information null mutant by deletion of ugt52 gene with no enzyme activity Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 114000
-
 cloned, calculated molecular mass Dictyostelium discoideum
2.4.1.173 136000
-
 cloned, calculated molecular mass Saccharomyces cerevisiae
2.4.1.173 136000
-
 cloned, calculated molecular mass Komagataella pastoris
2.4.1.173 171000
-
 cloned, calculated molecular mass Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Avena sativa O22678 ugt80A1
-
2.4.1.173 Avena sativa ugt80A1 O22678 ugt80A1
-
2.4.1.173 Candida albicans Q5A950 ugt51c1
-
2.4.1.173 Dictyostelium discoideum Q54IL5 ugt52
-
2.4.1.173 Dictyostelium discoideum ugt52 Q54IL5 ugt52
-
2.4.1.173 Komagataella pastoris
-
-
-
2.4.1.173 Komagataella pastoris Q9Y751 ugt51b1
-
2.4.1.173 Komagataella pastoris GS115 Q9Y751 ugt51b1
-
2.4.1.173 Saccharomyces cerevisiae Q06321 ugt51=ylr189C
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Dictyostelium discoideum ?
-
 ?
2.4.1.173 additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Komagataella pastoris ?
-
 ?
2.4.1.173 additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Saccharomyces cerevisiae ?
-
 ?
2.4.1.173 additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Avena sativa ?
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Dictyostelium discoideum UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Komagataella pastoris UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Saccharomyces cerevisiae UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Avena sativa ugt80A1 UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Dictyostelium discoideum ugt52 UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Komagataella pastoris GS115 UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Dictyostelium discoideum UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Komagataella pastoris UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Saccharomyces cerevisiae UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Avena sativa UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Avena sativa ugt80A1 UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Dictyostelium discoideum ugt52 UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Komagataella pastoris GS115 UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol
-
 Candida albicans UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Dictyostelium discoideum UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Komagataella pastoris UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Saccharomyces cerevisiae UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Avena sativa UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Avena sativa ugt80A1 UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Dictyostelium discoideum ugt52 UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Komagataella pastoris GS115 UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Dictyostelium discoideum UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Komagataella pastoris UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Saccharomyces cerevisiae UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Avena sativa UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Avena sativa ugt80A1 UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Dictyostelium discoideum ugt52 UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Komagataella pastoris GS115 UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Dictyostelium discoideum UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Komagataella pastoris UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Saccharomyces cerevisiae UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Avena sativa UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Avena sativa ugt80A1 UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Dictyostelium discoideum ugt52 UDP + tomatidine 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Komagataella pastoris GS115 UDP + tomatidine 3-beta-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.173 Ugt51
-
 Komagataella pastoris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.5
-
 Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Candida albicans
2.4.1.173 7.5
-
 Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Dictyostelium discoideum
2.4.1.173 7.5
-
 Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Komagataella pastoris
2.4.1.173 7.5
-
 Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Saccharomyces cerevisiae