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Literature summary extracted from

  • Forsee, W.T.; Laine, R.A.; Elbein, A.D.
    Solubilization of a particulate UDP-Glucose:sterol beta-glucosyltransferase in developing cotton fibers and seeds and characterization of steryl 6-acyl-D-glucosides (1974), Arch. Biochem. Biophys., 161, 248-259.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 lecithin stimulation by cotton and soybean lecithin but not with egg lecithin, appears to increase Vmax with sitosterol and Km of UDP-glucose Gossypium hirsutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 maleate buffer strong inhibition at 50 mM Gossypium hirsutum
2.4.1.173 phosphate buffer strong inhibition at 50 mM Gossypium hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.023
-
 UDP-glucose lecithin causes a 2fold increase in Km Gossypium hirsutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 membrane membrane fraction Gossypium hirsutum 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Gossypium hirsutum
-
 cotton
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation and DEAE-cellulose column Gossypium hirsutum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 cotton fiber
-
 Gossypium hirsutum
-
2.4.1.173 seed
-
 Gossypium hirsutum
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.173 2C, Tris-HCl 50 mM, pH 8.5, two weeks 75% of activity Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information UDP-glucose is an effective glucose donor but not so ADP-glucose, CDP-glucose, TDP-glucose, GDP-glucose, UDP-galactose or UDP-mannose Gossypium hirsutum ?
-
 ?
2.4.1.173 UDP-glucose + campesterol
-
 Gossypium hirsutum UDP + campesterol 3-beta-D-glucoside 4% of the products ?
2.4.1.173 UDP-glucose + sitosterol
-
 Gossypium hirsutum UDP + sitosterol 3-beta-D-glucoside 75% of the product ?
2.4.1.173 UDP-glucose + stigmasterol
-
 Gossypium hirsutum UDP + stigmasterol 3-beta-D-glucoside 6% of the product ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 37
-
 assay at Gossypium hirsutum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 8.5
-
 Tris-HCl 50 mM, Triton X-100 0.25% Gossypium hirsutum