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Literature summary extracted from

  • Paczkowski, C.; Zimowski, J.; Krawczyk, D.; Wojciechowski, Z.A.
    Steroid-specific glucosyltransferase in Asparagus plumosus shoots (1990), Phytochemistry, 29, 63-70.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 2-mercaptoethanol slightly stimulation Asparagus setaceus
2.4.1.173 p-chloromercuribenzoate potent inhibitory effect indicating that free SH groups are essential for activity Asparagus setaceus
2.4.1.173 Triton X-100 activity stimulated at 0.1-0.5% Asparagus setaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 p-chloromercuribenzoate potent inhibitory effect at 0.1 mM indicating that free SH groups are essential for activity Asparagus setaceus
2.4.1.173 UDP total inhibition at 1 mM Asparagus setaceus
2.4.1.173 UMP inhibition at 1 mM Asparagus setaceus
2.4.1.173 UTP total inhibition at 1 mM Asparagus setaceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 cytoplasm
-
 Asparagus setaceus 5737
-
2.4.1.173 membrane membrane fraction Asparagus setaceus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.173 Cu2+ inhibitory effect at 1 mM Asparagus setaceus
2.4.1.173 Hg2+ inhibitory effect at 1 mM Asparagus setaceus
2.4.1.173 Mg2+ not stimulated by 1 mM Asparagus setaceus
2.4.1.173 Mn2+ no effect at 1 mM Asparagus setaceus
2.4.1.173 additional information no metal ion requirement Asparagus setaceus
2.4.1.173 Zn2+ inhibitory effect at 1 mM Asparagus setaceus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Asparagus setaceus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 shoot
-
 Asparagus setaceus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information yamogenin is not substrate of this enzyme Asparagus setaceus ?
-
 ?
2.4.1.173 UDP-glucose + sitosterol
-
 Asparagus setaceus UDP + sitosterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Asparagus setaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.173 8.3
-
 Tris-HCl 0.1 M Asparagus setaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.173 7 8.7
-
 Asparagus setaceus