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Literature summary extracted from

  • Wojciechowski, Z.A.; Zimowski, J.; Zimowski, J.G.; Lyznik, A.
    Specificity of sterol-glucosylating enzymes from Sinapis alba and Physarum polycephalum (1979), Biochim. Biophys. Acta, 570, 363-370.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.173 synthesis
-
 Physarum polycephalum
2.4.1.173 synthesis synthesis of steryl beta-D-monoglucosides that are constituents of the cell membrane of higher plants Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 coprostanol potent inhibitor Physarum polycephalum
2.4.1.173 coprostanol not inhibitory Sinapis alba
2.4.1.173 epicholestanol potent inhibitor Physarum polycephalum
2.4.1.173 epicholestanol not inhibitory Sinapis alba
2.4.1.173 sodium deoxycholate
-
 Sinapis alba
2.4.1.173 sodium taurocholate
-
 Sinapis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.005
-
 sitosterol
-
 Sinapis alba
2.4.1.173 0.08
-
 UDP-glucose
-
 Sinapis alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 membrane
-
 Physarum polycephalum 16020
-
2.4.1.173 membrane organelles membrane Sinapis alba 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.173 Ca2+
-
 Physarum polycephalum
2.4.1.173 Ca2+ not stimulated between 0.001 mM to 10 mM Sinapis alba
2.4.1.173 Hg2+
-
 Physarum polycephalum
2.4.1.173 Hg2+ 50% of inhibition at 0.4 mM Sinapis alba
2.4.1.173 Mg2+
-
 Physarum polycephalum
2.4.1.173 Mg2+ not stimulated between 0.001 mM to 10 mM Sinapis alba
2.4.1.173 Zn2+
-
 Physarum polycephalum
2.4.1.173 Zn2+ 50% of inhibition at 0.006 mM Sinapis alba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 140000
-
 by gel filtration Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Physarum polycephalum
-
-
-
2.4.1.173 Sinapis alba
-
 white mustard
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation and Sephadex G-150 column Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 cell culture mycoplasma strain M3C IV Physarum polycephalum
-
2.4.1.173 seedling
-
 Sinapis alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information enzyme is absolute specific to UDP-glucose Sinapis alba ?
-
 ?
2.4.1.173 additional information glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates Physarum polycephalum ?
-
 ?
2.4.1.173 additional information glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates Sinapis alba ?
-
 ?
2.4.1.173 additional information enzyme can use CDP-glucose and TDP-glucose at 6 times slower rate Physarum polycephalum ?
-
 ?
2.4.1.173 additional information coprostanol and epicholestanol are not glucosylated Physarum polycephalum ?
-
 ?
2.4.1.173 additional information all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation Physarum polycephalum ?
-
 ?
2.4.1.173 additional information all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation Sinapis alba ?
-
 ?
2.4.1.173 UDP-glucose + campesterol
-
 Physarum polycephalum UDP + campesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + campesterol best substrate Sinapis alba UDP + campesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol
-
 Sinapis alba UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol best substrate Physarum polycephalum UDP + sitosterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Physarum polycephalum
2.4.1.173 30
-
 assay at Sinapis alba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.3
-
 Tris-HCl 0.1 M, Triton X-100 0.3% Sinapis alba