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Literature summary extracted from

  • Wojciechowski, J.; Zimowski, J.; Tyski, S.
    Enzymatic synthesis of steryl 3-beta-D-monoglucosides in the slime mold Physarum polycephalum (1977), Phytochemistry, 16, 911-914.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 2-mercaptoethanol 10% stimulation at 1 mM Physarum polycephalum
2.4.1.173 ATP 15% stimulation at 0.01 mM Physarum polycephalum
2.4.1.173 dithiothreitol 25% stimulation at 1 mM Physarum polycephalum
2.4.1.173 EDTA 15% stimulation at 0.1 mM Physarum polycephalum
2.4.1.173 lecithin 20-25% stimulation Physarum polycephalum

Application

EC Number Application Comment Organism
2.4.1.173 synthesis synthesis of steryl beta-D-monoglucosides possible function as glucose carriers through the membrane and intercellular transport of sterols Physarum polycephalum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 p-chloromercuribenzoate 50% inhibition at 0.60 mM Physarum polycephalum
2.4.1.173 Triton X-100 strong inhibition at concentrations below 0.05% Physarum polycephalum
2.4.1.173 Tween 20 strong inhibition at concentrations below 0.05% Physarum polycephalum
2.4.1.173 Tween 60 strong inhibition at concentrations below 0.05% Physarum polycephalum
2.4.1.173 UDP 50% inhibition at 0.06 mM Physarum polycephalum
2.4.1.173 UTP 50% inhibition at 0.70 mM Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.0056
-
 sitosterol
-
 Physarum polycephalum
2.4.1.173 0.18
-
 UDP-glucose
-
 Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 cytoplasm 50% of the activity Physarum polycephalum 5737
-
2.4.1.173 membrane membrane bound 50% of the activity Physarum polycephalum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.173 Ca2+ slightly inhibition above 0.1 mM Physarum polycephalum
2.4.1.173 Hg2+ 50% inhibition at 0.07 mM Physarum polycephalum
2.4.1.173 Mg2+ slightly inhibition above 0.1 mM Physarum polycephalum
2.4.1.173 Zn2+ 50% inhibition at 0.05 mM Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 70000
-
 gel filtration Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation and gel filtration on Sephadex G-100 Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 cell culture mycoplasma strain M3C IV Physarum polycephalum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information DELTA5-sterols are glucosylated at higher rate than DELTA7-sterols Physarum polycephalum ?
-
 ?
2.4.1.173 additional information presence and localization of double bonds in the ring system exert a pronounced effect on the rate of glucosylation Physarum polycephalum ?
-
 ?
2.4.1.173 additional information the presence of a DELTA22 double bond decreases affinity of the sterol for the enzyme Physarum polycephalum ?
-
 ?
2.4.1.173 additional information sterols possessing an alkyl group at C-24 are better substrates than C27 sterols Physarum polycephalum ?
-
 ?
2.4.1.173 additional information stanols and sterols with conjugated double bonds in ring B are poor substrates Physarum polycephalum ?
-
 ?
2.4.1.173 UDP-glucose + 5-alpha-cholestanol 8% of the activity with sitosterol Physarum polycephalum UDP + 5-alpha-cholestanol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + 5-cholestenol 12% of the activity with sitosterol Physarum polycephalum UDP + 5-cholestenol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + 7-dehydrocholesterol 6% of the activity with sitosterol Physarum polycephalum UDP + 7-dehydrocholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + alpha-spinasterol 31% of the activity with sitosterol Physarum polycephalum UDP + alpha-spinasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + campesterol 28% of the activity with sitosterol Physarum polycephalum UDP + campesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol 23% of the activity with sitosterol Physarum polycephalum UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol 11% of the activity with sitosterol Physarum polycephalum UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol greater activity than with other sterols Physarum polycephalum UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol 50% of the activity with sitosterol Physarum polycephalum UDP + stigmasterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.173 7.2
-
-
 Physarum polycephalum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.2
-
 Tris-HCl 0.1 M Physarum polycephalum