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Literature summary extracted from

  • Vinitsky, A.; Grubmeyer, C.
    A new paradigm for biochemical energy coupling. Salmonella typhimurium nicotinate phosphoribosyltransferase (1993), J. Biol. Chem., 268, 26004-26010.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.21 expression of pncB gene on a multicopy pUC19 plasmid in Salmonella typhimurium Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.21 0.0015
-
 nicotinate pncB gene, in the coupled reaction Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 0.022
-
 5-phospho-alpha-D-ribose 1-diphosphate pncB gene, in the coupled reaction Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 0.29
-
 nicotinate pncB gene, in the uncoupled reaction Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 4.5
-
 5-phospho-alpha-D-ribose 1-diphosphate pncB gene, in the uncoupled reaction Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.21 soluble
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.21 45000
-
 pncB gene, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.21 bacterial suspension disruption and centrifugation, chromatography on Sephadex-G, DEAE-Toyopearl, Cibacron Blue and Sephacryl gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.21 3
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
6.3.4.21 4ºC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 t1/2: at least 2 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
6.3.4.21 monomer
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
6.3.4.21 NAPRTase
-
-
6.3.4.21 nicotinate phosphoribosyltransferase
-
-
6.3.4.21 nicotinic acid phosphoribosyltransferase
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.21 ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Salmonella enterica subsp. enterica serovar Typhimurium