EC Number | General Stability | Organism |
---|---|---|
6.3.4.21 | first order activity loss in the presence of 1/200 trypsin/enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
6.3.4.21 | rapidly inactivates the ATPase and nicotinic acid mononucleotide synthesis activities with cleavages at Arg-384 and Lys-374, ATP and 5-phospho-alpha-D-ribose 1-diphosphate increase the t1/2 for inactivation 8fold and 4fold, respectively, and 32fold, together | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.21 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.21 | bacterial suspension disruption and centrifugation, chromatography on Q-Sepharose and phenyl-Sepharose | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.4.21 | 4ΒΊC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.21 | nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.21 | NAPRTase | - |
- |
6.3.4.21 | nicotinic acid phosphoribosyltransferase | - |
- |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.21 | ATP | 2000fold increase in the Kcat/KM | Salmonella enterica subsp. enterica serovar Typhimurium |