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Literature summary extracted from

  • Niedel, J.; Dietrich, L.S.
    Nicotinate phosphoribosyltransferase of human erythrocytes. Purification and properties (1973), J. Biol. Chem., 248, 3500-3505.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.21 50% activity recovered at salt concentrations of 50 mM, inactivation with salt concentrations below 20 mM, 20-35% glycerol partially protects Homo sapiens
6.3.4.21 glycerol, 20-35%, stabilizes during dialysis against low buffer concentrations, more highly purified preparations require higher glycerol concentrations Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.21 0.0005
-
 nicotinate in the presence of ATP Homo sapiens
6.3.4.21 0.024
-
 nicotinate reduced to 0.0005 mM in the presence of ATP Homo sapiens
6.3.4.21 0.06 0.1 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens
6.3.4.21 0.1
-
 5-phospho-alpha-D-ribose 1-diphosphate 0.005 mM in the presence of ATP Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.21 86000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Homo sapiens first step in NAD+-(salvage) biosynthesis beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.21 ammonium sulfate fractionation, chromatography on DEAE-cellulose, chromatography on Sephadex, affinity column chromatography and hydroxylapatite column chromatography, 30000fold purified Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.21 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.21 0.052
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.3.4.21 -70°C, t1/2: 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 additional information one mol ATP is cleaved per mol product formed Homo sapiens ?
-
 ?
6.3.4.21 additional information no ATPase activity in the absence of substrates Homo sapiens ?
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Homo sapiens beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O first step in NAD+-(salvage) biosynthesis Homo sapiens beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.21 nicotinate phosphoribosyltransferase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.21 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.21 6.5 8 broad, 0.2 M potassium phosphate buffer or 0.05 M Tris-Cl Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.4.21 5 10 stable, 0.2 M potassium phosphate buffer Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.21 ATP activation Homo sapiens
6.3.4.21 ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Homo sapiens