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Literature summary extracted from
- Biosynthesis of diphosphopyridine nucleotide. III. Nicotinic acid mononucleotide pyrophosphorylase (1961), J. Biol. Chem., 236, 525-530.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.21 | additional information | no activation by ADP | Bos taurus | |
| 6.3.4.21 | additional information | 3,5-cycloadenosine, borate and sulfate do not affect the reaction rate | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.21 | diphosphate | - |
Bos taurus |  |
| 6.3.4.21 | Mg2+ | at higher concentrations than ATP, when free Mg2+ ions occur | Bos taurus | |
| 6.3.4.21 | additional information | no inhibition by nicotinamide; no inhibition by nicotinate, up to 10 mM | Bos taurus | |
| 6.3.4.21 | nicotinate mononucleotide | competitive to both substrates | Bos taurus | |
| 6.3.4.21 | tripolyphosphate | - |
Bos taurus | |
| 6.3.4.21 | UTP | weak | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.3.4.21 | 0.001 | - |
nicotinate | - |
Bos taurus | |
| 6.3.4.21 | 0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 6.3.4.21 | soluble | - |
Bos taurus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.21 | Mg2+ | requirement | Bos taurus | |
| 6.3.4.21 | Mg2+ | synergism with ATP | Bos taurus | |
| 6.3.4.21 | MgATP2- | maximal activation at equimolar levels of Mg2+ and ATP, presumably enzyme structure stabilizing | Bos taurus | |
| 6.3.4.21 | additional information | no activation by borate, sulfate | Bos taurus | |
| 6.3.4.21 | phosphate | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.21 | nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | Bos taurus | - |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.4.21 | Bos taurus | - |
beef | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.4.21 | extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.3.4.21 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.21 | 0.0012 | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 6.3.4.21 | -12°C, partially purified enzyme preparation, t1/2: at least 3 months | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.21 | nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Bos taurus | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
| 6.3.4.21 | nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Bos taurus | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.3.4.21 | nicotinic acid mononucleotide pyrophosphorylase | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.21 | 37 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.21 | 7.2 | - |
broad, phosphorolysis | Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.21 | additional information | - |
active over a wide range | Bos taurus |
| 6.3.4.21 | 5.5 | 8 | about half-maximal activity at pH 5.5 and about 85% of maximal activity at pH 8 | Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.21 | 6.5 | - |
inactivation below, phosphorolysis | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.21 | ATP | activation | Bos taurus | |
| 6.3.4.21 | ATP | 60-70% stimulation with different enzyme preparations, the largest effects are observed with aged preparations of the enzyme | Bos taurus | |
| 6.3.4.21 | ATP | together with Mg2+ | Bos taurus | |
| 6.3.4.21 | CTP | 20-30% as effective as ATP | Bos taurus | |
| 6.3.4.21 | GTP | 20-30% as effective as ATP | Bos taurus | |
| 6.3.4.21 | ITP | 20-30% as effective as ATP | Bos taurus | |
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Release 2023.1 (January 2023)
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