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Literature summary extracted from

  • Cho, Y.; Sharma, V.; Sacchettini, J.C.
    Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 8333-8339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 hanging drop vapour diffusion method at 16°C, the apocrystals are obtained using 0.1 M buffer MES pH 6.5 and magnesium sulfate as precipitant, crystals in the presence of AMP and histidine are obtained using 0.1 M sodium citrate pH 5.6, 0.5 M ammonium sulfate and 1 M lithium sulfate with 5 mM AMP and 0.1 mM histidine Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 L-histidine allosteric inhibition, synergistically favored by AMP; feed-back inhibition Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 nickel-nitrilotriacetic acid column, Sephadex G-200 later on Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.17 dimer active form Mycobacterium tuberculosis
2.4.2.17 hexamer inactive form, in complex with histidine Mycobacterium tuberculosis