EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.17 | vapour diffusion method, trigonal prisms are obtained using 1.3 M sodium tartrate, 50-200 mM magnesium chloride, 100 mM citrate buffer pH 5.6 and enzyme in the presence of 2 mM AMP, round shaped crystals are obtained with 1.36-1.44 M ammonium sulfate, 0-0.3 M sodium chloride, 100 mM HEPES buffer pH 7.5 and enzyme in the presence of 2 mM AMP | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.17 | L-histidine | feed-back inhibition | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.17 | 33367 | - |
6 * 33367, dimers arranged in a hexamer, in the presence of AMP | Escherichia coli |
2.4.2.17 | 186000 | - |
dynamic light scattering in the presence of AMP or histidine | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.17 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.17 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.17 | centrifugation and DEAE-Sephacel anion-exchange column | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.17 | -20°C, 50 mM Tris-HCl, pH 7.5, 0.4 mM DTT and one protease inhibitor per litre, 50% v/v glycerol, long term storage | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.17 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
2.4.2.17 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Escherichia coli | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.17 | hexamer | 6 * 33367, dimers arranged in a hexamer, in the presence of AMP | Escherichia coli |