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Literature summary extracted from

  • Kryvi, H.
    Thermal stability of phosphoribosyladenosine triphosphate synthetase as reflected in its circular dichroism and activity properties. Effect of inhibitors (1973), Biochim. Biophys. Acta, 317, 123-130.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.17 histidine or AMP stabilizes the enzyme with respect to thermal inactivation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP linear competitive inhibitor with respect to ATP, stabilizes the enzyme to thermal inactivation, protect the ordered enzymatic structure against thermodenaturation Escherichia coli
2.4.2.17 L-histidine feed-back inhibition; stabilizes the enzyme to thermal inactivation, protects the ordered enzymatic structure against thermodenaturation, no interaction with binding sites Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli first step in histidine biosynthesis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Escherichia coli diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.17 additional information
-
 heat inactivation depends on protein concentration and inhibitors Escherichia coli
2.4.2.17 47.5
-
 1.9 mg enzyme/ml, 80 min, 75% loss of activity without addition of AMP, with 0.05 mM AMP about 60% loss of activity, with 0.5 mM AMP about 40% loss of activity, with 5 mM AMP about 25% loss of activity Escherichia coli
2.4.2.17 48
-
 3 mg enzyme/ml, 10 min, 15% remaining activity without addition of histidine, with 0.000086 mM histidine about 15% remaining activity after 15 min, with 0.00069 mM histidine about 70% remaining activity after 80 min, with 0.00345 mM histidine about 80% remaining activity after 80 min, with 0.0069 mM histidine about 55% remaining activity after 50 min Escherichia coli