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Literature summary extracted from

  • Vo, L.; Lee, S.; Marcinko, M.C.; Holmes, E.H.; Macher, B.A.
    Human alpha1,3/4-fucosyltransferases II. A single amino acid at the COOH terminus of FucT III and V alters their kinetic properties (1998), J. Biol. Chem., 273, 25250-25255.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.65 D336A fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V Homo sapiens
2.4.1.152 A349D mutant enzyme shows higher activity with a range of acceptor substrates, higher affinity for Fucalpha(1,2)Galbeta(1,4)GlcNAc, 8fold higher overall catalytic efficiency than that of wild-type enzyme. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.65 0.0336
-
 GDP-L-fucose fucosyltransferase III Homo sapiens
2.4.1.65 0.1
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens
2.4.1.65 0.1145
-
 GDP-D-fucose mutant enzyme D336A Homo sapiens
2.4.1.65 3.8
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III mutant enzyme D336A Homo sapiens
2.4.1.152 0.0188
-
 GDP-L-fucose fucosyltransferase V Homo sapiens
2.4.1.152 0.0233
-
 GDP-L-fucose fucosyltransferase V mutant enzyme A349D Homo sapiens
2.4.1.152 1.1
-
 Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V mutant enzyme A349D Homo sapiens
2.4.1.152 3.9
-
 Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
 fucosyltransferase III
-
2.4.1.152 Homo sapiens
-
 fucosyltransferase V
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-D-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme D336A shows 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)GlcNAc
-
 Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
 ?
2.4.1.152 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme A349D shows 8fold higher activity than the wild-type enzyme Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.65 3.02
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III mutant enzyme D336A Homo sapiens
2.4.1.65 8.02
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III Homo sapiens
2.4.1.152 1.7
-
 Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V Homo sapiens
2.4.1.152 3.6
-
 Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V mutant enzyme A349D Homo sapiens