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Literature summary extracted from

  • Sousa, V.L.; Costa, M.T.; Palma, A.S.; Enguita, F.; Costa, J.
    Localization, purification and specificity of the full-length membrane-bound form of human recombinant alpha 1,3/4-fucosyltransferase from BHK-21B cells (2001), Biochem. J., 357, 803-810.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.65 expression in BHK-21B cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.65 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.65 Golgi trans-face Golgi type II membrane protein Homo sapiens
-
-
2.4.1.65 membrane
-
 Homo sapiens 16020
-
2.4.1.65 soluble
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.65 GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R Homo sapiens the enzyme catalyzes the synthesis of fucosylated Lewis motifs that are associated with cell-adhesion events and are differentially expressed during cell differentiation GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.65
-
 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.65 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R the enzyme has a clear preference for the Galbeta3GlcNAc motif in oligosaccharides conjugated with the hydrophobic tail (CH2)3-NHCO-(CH2)5-NH-biotin Homo sapiens GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.65 GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R the enzyme catalyzes the synthesis of fucosylated Lewis motifs that are associated with cell-adhesion events and are differentially expressed during cell differentiation Homo sapiens GDP + 1,3-beta-D-galactosyl-(alpha-(1,4)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.65 GDP-L-fucose + asialofetuin no activity Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin 193% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-(CH2)5-NH-biotin 9.3% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-(CH2)5-NH-biotin
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 ?
2.4.1.65 GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAc-(CH2)5-NH-biotin 57% of the activity with Galbeta(1,3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin Homo sapiens GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-(CH2)5-NH-biotin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.65 More the enzyme is present in an equilibrium of monomer/dimer in the trans-Golgi/trans-Golgi-network of transfected BHK cells Homo sapiens