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Literature summary extracted from

  • Palma, A.S.; Vila-Verde, C.; Pires, A.S.; Fevereiro, P.S.; Costa, J.
    A novel plant alpha4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewisa adhesion determinant (2001), FEBS Lett., 499, 235-238.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.65 Co2+ 20 mM, 98% inhibition Vaccinium myrtillus
2.4.1.65 Cu2+ 20 mM, complete inhibition Vaccinium myrtillus
2.4.1.65 Mn2+ 20 mM, 40% inhibition below pH 8.0 Vaccinium myrtillus
2.4.1.65 Zn2+ 20 mM, complete inhibition Vaccinium myrtillus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.65 microsome
-
 Vaccinium myrtillus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.65 Mn2+
-
 Homo sapiens
2.4.1.65 Mn2+ activation above pH 8.0 Vaccinium myrtillus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
-
-
2.4.1.65 Vaccinium myrtillus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.65 cell suspension culture
-
 Vaccinium myrtillus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
-
 Homo sapiens GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.65 GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R transfer of fucose to N-acetylglucosamine in the type I Galbeta3GlcNAc motif from oligosaccharides linked to a hydrophobic tail and glycoproteins containing the type I motif. Sialylated oligosaccharides containing the type II Galbeta4GlcNAc motif are not acceptors Vaccinium myrtillus GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.65 GDP-L-fucose + asialofetuin
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + asialofetuin 7.3% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-O-sp-biotin 118% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-O-sp-biotin 10.5% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O-sp-biotin
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)GlcNAc-O-sp-biotin
-
 Vaccinium myrtillus GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.65 40
-
-
 Vaccinium myrtillus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.65 25 50 25°C: about 50% of maximal activity, 50°C: about 40% of maximal activity Vaccinium myrtillus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.65 50
-
 stable up to Vaccinium myrtillus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.65 7
-
 both in the presence and in absence of Mn2+ Vaccinium myrtillus