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Literature summary extracted from

  • Takahashi, I.; Ogura, K.; Seto, S.
    Heptaprenyl pyrophosphate synthetase from Bacillus subtilis (1980), J. Biol. Chem., 255, 4539-4543.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.30 0.0083
-
 geranylgeranyl diphosphate pH 7.5, 37°C Bacillus subtilis
2.5.1.30 0.0128
-
 isopentenyl diphosphate pH 7.5, 37°C Bacillus subtilis
2.5.1.30 0.0133
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.30 Mg2+ required, no activation with Mn2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.30 45000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.30 Bacillus subtilis P31112 and P31114 P31112: subunit 1, P31114: subunit 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.30 partial Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diposphate without accumulation of any prenyl diphosphate shorter than C35 Bacillus subtilis 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 geranylgeranyl diphosphate + 3 isopentenyl diphosphate the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diphosphate without accumulation of any prenyl diphosphate shorter than C35. Poor activity of geranylneryl diphosphate contrast with the high activity of the all-trans isomer is consistent with the proposal that this enzyme catalyzes condensations resulting in trans products Bacillus subtilis 3 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 additional information the enzyme does not catalyze a reaction between isopentenyl diphosphate and either dimethylallyl or geranyl diphosphate Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.30 heptaprenyl pyrophosphate synthetase
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.30 6.5 7
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.30 5.5 8 pH 5.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity Bacillus subtilis