Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • G๖tting, C.; Kuhn, J.; Zahn, R.; Brinkmann, T.; Kleesiek, K.
    Molecular cloning and expression of human UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase and its first isoform XT-II (2000), J. Mol. Biol., 304, 517-528.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.26 DNA sequence determination and analysis of isoforms XT-I and XT-II, chromosome mapping Rattus norvegicus
2.4.2.26 DNA sequence determination and analysis, chromosome mapping, functional expression of isoforms XT-I and XT-II in CHO-K1 cells, no activity when XT-II is expressed fused to the aminoterminal peptide tag, 8% of the recombinant activity is located in the cytosol and membranes, 92% recombinant activity is secreted into the medium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.26 heparin
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.26 extracellular in cell culture supernatant Homo sapiens
-
-
2.4.2.26 extracellular isoform XT-I Homo sapiens
-
-
2.4.2.26 membrane isoform XT-II, type II transmembrane protein Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.26 91000
-
 x * 91000, isoform XT-I, amino acid sequence determination Homo sapiens
2.4.2.26 97000
-
 x * 97000, isoform XT-II, amino acid sequence determination Homo sapiens
2.4.2.26 120000
-
 gel filtration, amino acid sequence determination Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Homo sapiens
-
 2 isoforms XT-I and XT-II
-
2.4.2.26 Rattus norvegicus
-
 2 isoforms XT-I and XT-II
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.2.26 glycoprotein 3 potential N-glycosylation sites, isoforms XT-I and XT-II Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.26 wild-type, and recombinant from CHO-K1 cells Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine = UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine isoform XT-I contains the DxD motif at position 183, which has shown to be essential for binding of nucleotide sugars in glycosyltransferases Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.26 brain
-
 Rattus norvegicus
-
2.4.2.26 brain low content of isoform XT-II, and very low content of isoform XT-I Homo sapiens
-
2.4.2.26 choriocarcinoma cell
-
 Homo sapiens
-
2.4.2.26 heart
-
 Rattus norvegicus
-
2.4.2.26 heart isoform XT-II, low content Homo sapiens
-
2.4.2.26 JAR cell choriocarcinoma cell line Homo sapiens
-
2.4.2.26 kidney
-
 Rattus norvegicus
-
2.4.2.26 kidney isoforms XT-I and XT-II Homo sapiens
-
2.4.2.26 liver
-
 Rattus norvegicus
-
2.4.2.26 liver isoform XT-II Homo sapiens
-
2.4.2.26 lung
-
 Rattus norvegicus
-
2.4.2.26 lung isoform XT-II, and low content of isoform XT-I Homo sapiens
-
2.4.2.26 pancreas isoforms XT-I and XT-II Homo sapiens
-
2.4.2.26 placenta isoform XT-I and XT-II Homo sapiens
-
2.4.2.26 skeletal muscle isoform XT-II, low content Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Homo sapiens UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.26 ? x * 91000, isoform XT-I, amino acid sequence determination Homo sapiens
2.4.2.26 ? x * 97000, isoform XT-II, amino acid sequence determination Homo sapiens
2.4.2.26 monomer
-
 Rattus norvegicus
2.4.2.26 monomer 1 * 120000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 XT
-
 Rattus norvegicus
2.4.2.26 XT 2 isoforms XT-I and XT-II Homo sapiens