BRENDA - Enzyme Database

Recognition of acceptor proteins by UDP-D-xylose proteoglycan core protein beta-D-xylosyltransferase

Brinkmann, T.; Weilke, C.; Kleesiek, K.; J. Biol. Chem. 272, 11171-11175 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.26
0.0006
-
bikunin
recombinant mutant bikunin
Homo sapiens
2.4.2.26
0.0009
-
bikunin
recombinant wild-type bikunin
Homo sapiens
2.4.2.26
0.022
-
QEEEGSGGGOK
-
Homo sapiens
2.4.2.26
0.13
-
NFDEIDRSGFGFN
-
Homo sapiens
2.4.2.26
0.155
-
cartilage chondroitin sulfate proteoglycan
trifluoromethanesulfonic acid-degraded substrate
Homo sapiens
2.4.2.26
0.19
-
cartilage chondroitin sulfate proteoglycan
hydrogen fluoride-degraded substrate
Homo sapiens
2.4.2.26
0.39
-
PLVSSGEDEPK
-
Homo sapiens
2.4.2.26
0.42
-
WAGGDASGE
-
Homo sapiens
2.4.2.26
0.545
-
silk fibroin
-
Homo sapiens
2.4.2.26
0.94
-
VCRSGSGLVGK
-
Homo sapiens
2.4.2.26
2.67
-
GVEGSADFLK
-
Homo sapiens
2.4.2.26
3.625
-
KTKGSGFFVF
-
Homo sapiens
2.4.2.26
8.27
-
KKDSGPY
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.26
extracellular
in cell culture supernatant
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
Homo sapiens
initiation of chondroitin sulfate biosynthesis
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.26
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.4.2.26
partial
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine = UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
chondroitin sulfate attachment site, determination of the consensus sequence for the recognition signal of the enzyme
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.26
cartilage
sternal, from heart
Homo sapiens
-
2.4.2.26
chondrocyte
from heart sternal cartilage
Homo sapiens
-
2.4.2.26
chondrocyte
cell culture supernatant
Homo sapiens
-
2.4.2.26
heart
sternal cartilage
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.26
additional information
very low activity with tripeptide SGG and peptide LNFSTGW
637615
Homo sapiens
?
-
-
-
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
637615
Homo sapiens
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
transfers a beta-D-xylosyl residue to specific serine residues dependent on the consensus signal sequence
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
637615
Homo sapiens
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
initiation of chondroitin sulfate biosynthesis
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
-
-
?
2.4.2.26
UDP-D-xylose + bikunin
recombinant wild-type from Chang liver hepatocytes and mutant, expressed in Escherichia coli
637615
Homo sapiens
UDP + D-xylosyl-bikunin
bound to serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + cartilage chondroitin sulfate proteoglycan
degraded by hydrogen fluoride or trifluromethanesulfonic acid
637615
Homo sapiens
UDP + cartilage chondroitin sulfate proteoglycan with xylosylserine
-
-
-
?
2.4.2.26
UDP-D-xylose + GVEGSADFLK
derived from collagen X
637615
Homo sapiens
UDP + GVEGS(-D-xylose)ADFLK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + KKDSGPY
-
637615
Homo sapiens
UDP + KKDS(-D-xylose)GPY
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + KTKGSGFFVF
-
637615
Homo sapiens
UDP + KTKGS(-D-xylose)GFFVF
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + NFDEIDRSGFGFN
-
637615
Homo sapiens
UDP + NFDEIDRS(-D-xylose)GFGFN
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + PLVSSGEDEPK
derived from neurocan protein
637615
Homo sapiens
UDP + ?
D-xylose bound to a serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + QEEEGSGGGOK
best substrate
637615
Homo sapiens
UDP + QEEEGS(-D-xylose)GGGOK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + QEEEGSGGGOK
peptide derived from bikunin
637615
Homo sapiens
UDP + QEEEGS(-D-xylose)GGGOK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + silk fibroin
acceptor substrate contains repetitive sequence Gly-Ser-Gly-Ala-Gly-Ala
637615
Homo sapiens
UDP + silk fibroin with xylosylserine
-
-
-
?
2.4.2.26
UDP-D-xylose + VCRSGSGLVGK
derived from apolipoprotein J
637615
Homo sapiens
UDP + VCRSGSGLVGK
D-xylose bound to a serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + WAGGDASGE
-
637615
Homo sapiens
UDP + WAGGDAS(-D-xylose)GE
-
637615
Homo sapiens
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.26
XT
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.26
34
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.26
6.5
-
assay at
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.26
0.0006
-
bikunin
recombinant mutant bikunin
Homo sapiens
2.4.2.26
0.0009
-
bikunin
recombinant wild-type bikunin
Homo sapiens
2.4.2.26
0.022
-
QEEEGSGGGOK
-
Homo sapiens
2.4.2.26
0.13
-
NFDEIDRSGFGFN
-
Homo sapiens
2.4.2.26
0.155
-
cartilage chondroitin sulfate proteoglycan
trifluoromethanesulfonic acid-degraded substrate
Homo sapiens
2.4.2.26
0.19
-
cartilage chondroitin sulfate proteoglycan
hydrogen fluoride-degraded substrate
Homo sapiens
2.4.2.26
0.39
-
PLVSSGEDEPK
-
Homo sapiens
2.4.2.26
0.42
-
WAGGDASGE
-
Homo sapiens
2.4.2.26
0.545
-
silk fibroin
-
Homo sapiens
2.4.2.26
0.94
-
VCRSGSGLVGK
-
Homo sapiens
2.4.2.26
2.67
-
GVEGSADFLK
-
Homo sapiens
2.4.2.26
3.625
-
KTKGSGFFVF
-
Homo sapiens
2.4.2.26
8.27
-
KKDSGPY
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.26
extracellular
in cell culture supernatant
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
Homo sapiens
initiation of chondroitin sulfate biosynthesis
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.26
partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.26
cartilage
sternal, from heart
Homo sapiens
-
2.4.2.26
chondrocyte
from heart sternal cartilage
Homo sapiens
-
2.4.2.26
chondrocyte
cell culture supernatant
Homo sapiens
-
2.4.2.26
heart
sternal cartilage
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.26
additional information
very low activity with tripeptide SGG and peptide LNFSTGW
637615
Homo sapiens
?
-
-
-
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
637615
Homo sapiens
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
transfers a beta-D-xylosyl residue to specific serine residues dependent on the consensus signal sequence
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
637615
Homo sapiens
?
2.4.2.26
UDP-alpha-D-xylose + [protein]-L-serine
initiation of chondroitin sulfate biosynthesis
637615
Homo sapiens
UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
-
-
?
2.4.2.26
UDP-D-xylose + bikunin
recombinant wild-type from Chang liver hepatocytes and mutant, expressed in Escherichia coli
637615
Homo sapiens
UDP + D-xylosyl-bikunin
bound to serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + cartilage chondroitin sulfate proteoglycan
degraded by hydrogen fluoride or trifluromethanesulfonic acid
637615
Homo sapiens
UDP + cartilage chondroitin sulfate proteoglycan with xylosylserine
-
-
-
?
2.4.2.26
UDP-D-xylose + GVEGSADFLK
derived from collagen X
637615
Homo sapiens
UDP + GVEGS(-D-xylose)ADFLK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + KKDSGPY
-
637615
Homo sapiens
UDP + KKDS(-D-xylose)GPY
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + KTKGSGFFVF
-
637615
Homo sapiens
UDP + KTKGS(-D-xylose)GFFVF
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + NFDEIDRSGFGFN
-
637615
Homo sapiens
UDP + NFDEIDRS(-D-xylose)GFGFN
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + PLVSSGEDEPK
derived from neurocan protein
637615
Homo sapiens
UDP + ?
D-xylose bound to a serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + QEEEGSGGGOK
best substrate
637615
Homo sapiens
UDP + QEEEGS(-D-xylose)GGGOK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + QEEEGSGGGOK
peptide derived from bikunin
637615
Homo sapiens
UDP + QEEEGS(-D-xylose)GGGOK
-
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + silk fibroin
acceptor substrate contains repetitive sequence Gly-Ser-Gly-Ala-Gly-Ala
637615
Homo sapiens
UDP + silk fibroin with xylosylserine
-
-
-
?
2.4.2.26
UDP-D-xylose + VCRSGSGLVGK
derived from apolipoprotein J
637615
Homo sapiens
UDP + VCRSGSGLVGK
D-xylose bound to a serine residue
637615
Homo sapiens
?
2.4.2.26
UDP-D-xylose + WAGGDASGE
-
637615
Homo sapiens
UDP + WAGGDAS(-D-xylose)GE
-
637615
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.26
34
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.26
6.5
-
assay at
Homo sapiens