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Literature summary extracted from

  • Du, W.; Brown, J.R.; Sylvester, D.R.; Huang, J.; Chalker, A.F.; So, C.Y.; Holmes, D.J.; Payne, D.J.; Wallis, N.G.
    Two active forms of UDP-N-acetylglucosamine enolpyruvyl transferase in gram-positive bacteria (2000), J. Bacteriol., 182, 4146-4152.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 genes murA1 and murA2, DNA and amino acid sequence determination, overexpression of both isoforms in Escherichia coli BL21(DE3) Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.7 additional information individually inactivation of the 2 isoforms by allelic replacement shows, that the 2 forms can substitute for each other, a double deletion mutant is not viable Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 0.011
-
 phosphoenolpyruvate isoform MurA2 Streptococcus pneumoniae
2.5.1.7 0.037
-
 phosphoenolpyruvate isoform MurA1 Streptococcus pneumoniae
2.5.1.7 0.12
-
 UDP-GlcNAc isoform MurA2 Streptococcus pneumoniae
2.5.1.7 0.244
-
 UDP-GlcNAc isoform MurA1 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Streptococcus pneumoniae enzyme activity is essential for the organism, the 2 isoforms can substitute for each other phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Streptococcus pneumoniae
-
-
-
2.5.1.7 Streptococcus pneumoniae
-
 2 genes murA1 and murA2, 2 isoforms of enzyme MurA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 recombinant, 2 isoforms from Escherichia coli Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Streptococcus pneumoniae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine enzyme activity is essential for the organism, the 2 isoforms can substitute for each other Streptococcus pneumoniae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 More phylogenetic tree Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.7 0.41
-
 UDP-GlcNAc isoform MurA1 Streptococcus pneumoniae
2.5.1.7 0.78
-
 UDP-GlcNAc isoform MurA2 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.7 7.5
-
 assay at Streptococcus pneumoniae