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Literature summary extracted from

  • Samland, A.K.; Amrhein, N.; Macheroux, P.
    Lysine 22 in UDP-N-acetylglucosamine enolpyruvyl transferase from Enterobacter cloacae is crucial for enzymatic activity and the formation of covalent adducts with the substrate phosphoenolpyruvate and the antibiotic fosfomycin (1999), Biochemistry, 38, 13162-13169.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.7 dithiothreitol included in the assay reaction mixture Enterobacter cloacae

Application

EC Number Application Comment Organism
2.5.1.7 medicine enzyme is a target for the antibiotic fosfomycin Enterobacter cloacae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 expression of wild-type and mutants in Escherichia coli Enterobacter cloacae

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.7 C115S
-
Enterobacter cloacae
2.5.1.7 K22E site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, altered UDP-GlcNAc binding, highly reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin Enterobacter cloacae
2.5.1.7 K22R site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, slightly reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin Enterobacter cloacae
2.5.1.7 K22V site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin Enterobacter cloacae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 fosfomycin
-
Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Enterobacter cloacae P33038 MurA
-
2.5.1.7 Enterobacter cloacae DSM 30054 P33038 MurA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 recombinant wild-type and mutants from Escherichia coli Enterobacter cloacae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine Lys22 is located near the active site and involved in substrate binding Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.7 additional information
-
activity of K22 mutants compared to wild-type in forward and reverse reaction, coupled assay Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine substrate binding structure Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine thermodynamic parameters of substrate binding, wild-type, C115S and K22 mutants Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine substrate binding structure Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine thermodynamic parameters of substrate binding, wild-type, C115S and K22 mutants Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
r

Subunits

EC Number Subunits Comment Organism
2.5.1.7 More structure Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.7 25
-
assay at Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.7 7.4
-
assay at Enterobacter cloacae