Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, D.H.; Lees, W.J.; Kempsell, K.E.; Lane, W.S.; Duncan, K.; Walsh, C.T.
    Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin (1996), Biochemistry, 35, 4923-4928.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.7 C115A site-directed mutagenesis, overexpression in Escherichia coli, no activity Escherichia coli
2.5.1.7 C115D site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, forms only the phospholactyl-enzyme intermediate adduct, but no UDP-GlcNAc-phosphoenolpyruvate, higher kcat than the wild-type at pH 7.0, enhanced pH-dependency of the reaction Escherichia coli
2.5.1.7 C115E site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, enhanced pH-dependency of the reaction, low activity Escherichia coli
2.5.1.7 C115N site-directed mutagenesis, overexpression in Escherichia coli, deamination of Asn115 to Asp115 Escherichia coli
2.5.1.7 C115S
-
Enterobacter cloacae
2.5.1.7 C115S site-directed mutagenesis, overexpression in Escherichia coli, no activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 (Z)-3-fluorophosphoenolpyruvate kinetics, mutant C115D; wild-type and mutant C115D, competitive against phosphoenolpyruvate Escherichia coli
2.5.1.7 fosfomycin alkylates Cys115 Enterobacter cloacae
2.5.1.7 fosfomycin alkylates Cys115; competitive against phosphoenolpyruvate; inhibits the wild-type, mutant C155D is completely resistant; t1/2 for inactivation of the wild-type enzyme: 6 s Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 0.0004
-
phosphoenolpyruvate wild-type enzyme Escherichia coli
2.5.1.7 0.015
-
UDP-GlcNAc wild-type enzyme Escherichia coli
2.5.1.7 0.016
-
UDP-GlcNAc mutant C115D, pH 8.0 Escherichia coli
2.5.1.7 0.021
-
UDP-GlcNAc mutant C115D, pH 6.0 Escherichia coli
2.5.1.7 0.022
-
phosphoenolpyruvate mutant C115D, pH 6.0 Escherichia coli
2.5.1.7 0.037
-
phosphoenolpyruvate mutant C115D, pH 8.0 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Enterobacter cloacae
-
-
-
2.5.1.7 Escherichia coli
-
MurA
-
2.5.1.7 Escherichia coli MurA
-
MurA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine Cys115 is the active site nucleophile Escherichia coli
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine Cys115 is the active site nucleophile Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Escherichia coli phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Escherichia coli MurA phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 UDP-GlcNAc enolpyruvyl transferase
-
Escherichia coli
2.5.1.7 UDP-GlcNAc enolpyruvyl transferase
-
Enterobacter cloacae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.7 0.0086
-
fosfomycin wild-type enzyme Escherichia coli
2.5.1.7 0.04
-
(Z)-3-fluorophosphoenolpyruvate wild-type enzyme Escherichia coli
2.5.1.7 0.04
-
(Z)-3-fluorophosphoenolpyruvate at pH 8.0 Escherichia coli
2.5.1.7 0.08
-
(Z)-3-fluorophosphoenolpyruvate at pH 8.0 Escherichia coli
2.5.1.7 0.08
-
(Z)-3-fluorophosphoenolpyruvate mutant C115D Escherichia coli
2.5.1.7 0.1
-
(Z)-3-fluorophosphoenolpyruvate at pH 6.0 Escherichia coli
2.5.1.7 0.1
-
(Z)-3-fluorophosphoenolpyruvate mutant C115D Escherichia coli
2.5.1.7 1
-
fosfomycin at pH 8.0 Escherichia coli
2.5.1.7 1
-
fosfomycin mutant C115D Escherichia coli
2.5.1.7 2
-
fosfomycin at pH 6.0 Escherichia coli
2.5.1.7 2
-
fosfomycin mutant C115D Escherichia coli