EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.7 | C115A | site-directed mutagenesis, overexpression in Escherichia coli, no activity | Escherichia coli |
2.5.1.7 | C115D | site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, forms only the phospholactyl-enzyme intermediate adduct, but no UDP-GlcNAc-phosphoenolpyruvate, higher kcat than the wild-type at pH 7.0, enhanced pH-dependency of the reaction | Escherichia coli |
2.5.1.7 | C115E | site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, enhanced pH-dependency of the reaction, low activity | Escherichia coli |
2.5.1.7 | C115N | site-directed mutagenesis, overexpression in Escherichia coli, deamination of Asn115 to Asp115 | Escherichia coli |
2.5.1.7 | C115S | - |
Enterobacter cloacae |
2.5.1.7 | C115S | site-directed mutagenesis, overexpression in Escherichia coli, no activity | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.7 | (Z)-3-fluorophosphoenolpyruvate | kinetics, mutant C115D; wild-type and mutant C115D, competitive against phosphoenolpyruvate | Escherichia coli | |
2.5.1.7 | fosfomycin | alkylates Cys115 | Enterobacter cloacae | |
2.5.1.7 | fosfomycin | alkylates Cys115; competitive against phosphoenolpyruvate; inhibits the wild-type, mutant C155D is completely resistant; t1/2 for inactivation of the wild-type enzyme: 6 s | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.7 | 0.0004 | - |
phosphoenolpyruvate | wild-type enzyme | Escherichia coli | |
2.5.1.7 | 0.015 | - |
UDP-GlcNAc | wild-type enzyme | Escherichia coli | |
2.5.1.7 | 0.016 | - |
UDP-GlcNAc | mutant C115D, pH 8.0 | Escherichia coli | |
2.5.1.7 | 0.021 | - |
UDP-GlcNAc | mutant C115D, pH 6.0 | Escherichia coli | |
2.5.1.7 | 0.022 | - |
phosphoenolpyruvate | mutant C115D, pH 6.0 | Escherichia coli | |
2.5.1.7 | 0.037 | - |
phosphoenolpyruvate | mutant C115D, pH 8.0 | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.7 | Enterobacter cloacae | - |
- |
- |
2.5.1.7 | Escherichia coli | - |
MurA | - |
2.5.1.7 | Escherichia coli MurA | - |
MurA | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.7 | phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | Cys115 is the active site nucleophile | Escherichia coli | |
2.5.1.7 | phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | Cys115 is the active site nucleophile | Enterobacter cloacae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.7 | phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | ? | |
2.5.1.7 | phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | ? | |
2.5.1.7 | phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli MurA | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.7 | UDP-GlcNAc enolpyruvyl transferase | - |
Escherichia coli |
2.5.1.7 | UDP-GlcNAc enolpyruvyl transferase | - |
Enterobacter cloacae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.7 | 0.0086 | - |
fosfomycin | wild-type enzyme | Escherichia coli | |
2.5.1.7 | 0.04 | - |
(Z)-3-fluorophosphoenolpyruvate | wild-type enzyme | Escherichia coli | |
2.5.1.7 | 0.04 | - |
(Z)-3-fluorophosphoenolpyruvate | at pH 8.0 | Escherichia coli | |
2.5.1.7 | 0.08 | - |
(Z)-3-fluorophosphoenolpyruvate | at pH 8.0 | Escherichia coli | |
2.5.1.7 | 0.08 | - |
(Z)-3-fluorophosphoenolpyruvate | mutant C115D | Escherichia coli | |
2.5.1.7 | 0.1 | - |
(Z)-3-fluorophosphoenolpyruvate | at pH 6.0 | Escherichia coli | |
2.5.1.7 | 0.1 | - |
(Z)-3-fluorophosphoenolpyruvate | mutant C115D | Escherichia coli | |
2.5.1.7 | 1 | - |
fosfomycin | at pH 8.0 | Escherichia coli | |
2.5.1.7 | 1 | - |
fosfomycin | mutant C115D | Escherichia coli | |
2.5.1.7 | 2 | - |
fosfomycin | at pH 6.0 | Escherichia coli | |
2.5.1.7 | 2 | - |
fosfomycin | mutant C115D | Escherichia coli |