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Literature summary extracted from

  • Wanke, C.; Falchetto, R.; Amrhein, N.
    The UDP-N-acetylglucosamine 1-carboxyvinyl-transferase of Enterobacter cloacae. Molecular cloning, sequencing of the gene and overexpression of the enzyme (1992), FEBS Lett., 301, 271-276.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 DNA and amino acid sequence determination, functional overexpression in Escherichia coli strain JM105 Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.7 37000
-
 nondenaturing PAGE Enterobacter cloacae
2.5.1.7 42100
-
 42100, SDS-PAGE Enterobacter cloacae
2.5.1.7 44780
-
 MW deduced from amino acid sequence Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae DSM 30054 enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Enterobacter cloacae
-
 Nr. 30054
-
2.5.1.7 Enterobacter cloacae DSM 30054
-
 Nr. 30054
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.7 1.8
-
 purified native enzyme Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.7 monomer 42100, SDS-PAGE Enterobacter cloacae

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 enol-pyruvyltransferase
-
 Enterobacter cloacae