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Literature summary extracted from

  • Illarionov, B.; Kemter, K.; Eberhardt, S.; Richter, G.; Cushman, M.; Bacher, A.
    Riboflavin synthase of Escherichia coli. Effect of single amino acid substitutions on reaction rate and ligand binding properties (2001), J. Biol. Chem., 276, 11524-11530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.9 ribC hyperexpression strain, DNA sequence determination of mutants Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.9 D143G site-directed mutagenesis, soluble protein, too unstable to be purified Escherichia coli
2.5.1.9 D143N site-directed mutagenesis, soluble protein, too unstable to be purified Escherichia coli
2.5.1.9 D185L site-directed mutagenesis, low remaining activity Escherichia coli
2.5.1.9 E183G site-directed mutagenesis, reduced activity Escherichia coli
2.5.1.9 E66G site-directed mutagenesis, low remaining activity Escherichia coli
2.5.1.9 E85G site-directed mutagenesis, reduced activity Escherichia coli
2.5.1.9 F2A site-directed mutagenesis, no remaining activity Escherichia coli
2.5.1.9 F2Y site-directed mutagenesis, very low remaining activity Escherichia coli
2.5.1.9 H102Q site-directed mutagenesis, very low remaining activity Escherichia coli
2.5.1.9 H97Q site-directed mutagenesis, low remaining activity Escherichia coli
2.5.1.9 K137A site-directed mutagenesis, low remaining activity Escherichia coli
2.5.1.9 additional information 5 mutants genes cannot be expressed recombinantly in Escherichia coli: C48S, T50R, T67R, T148R, T165R Escherichia coli
2.5.1.9 additional information a F2DELTA deletion mutant construct has no remaining activity Escherichia coli
2.5.1.9 N181G site-directed mutagenesis, soluble protein, too unstable to be purified Escherichia coli
2.5.1.9 N45G site-directed mutagenesis, slightly reduced activity Escherichia coli
2.5.1.9 N83G site-directed mutagenesis, reduced activity Escherichia coli
2.5.1.9 S146G site-directed mutagenesis, low remaining activity Escherichia coli
2.5.1.9 S41A site-directed mutagenesis, very low remaining activity Escherichia coli
2.5.1.9 T3R site-directed mutagenesis, slightly reduced activity, low expression rate Escherichia coli
2.5.1.9 T71A site-directed mutagenesis, slightly reduced activity Escherichia coli
2.5.1.9 Y133A site-directed mutagenesis, soluble protein, too unstable to be purified Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Escherichia coli
-
 gene ribC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9 wild-type enzyme and mutants Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Escherichia coli
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine substrate binding site Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.9 More N-terminal structure, ligand binding site Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.9 0.00833
-
 6,7-dimethyl-8-ribityllumazine per subunit Escherichia coli