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Literature summary extracted from

  • Plaut, G.W.E.
    Studies on the nature of the enzymic conversion of 6,7-dimethyl-8-ribityllumazine to riboflavin (1963), J. Biol. Chem., 238, 2225-2243.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.9 2,3-dimercapto-1-propanol enhances activity Saccharomyces cerevisiae
2.5.1.9 2,3-dimercapto-1-propanol enhances activity Eremothecium gossypii
2.5.1.9 2-mercaptoethanol enhances activity Saccharomyces cerevisiae
2.5.1.9 2-mercaptoethanol enhances activity Eremothecium gossypii
2.5.1.9 N-acetylcysteine enhances activity Saccharomyces cerevisiae
2.5.1.9 N-acetylcysteine enhances activity Eremothecium gossypii
2.5.1.9 SO32- enhances activity Saccharomyces cerevisiae
2.5.1.9 SO32- enhances activity Eremothecium gossypii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.9 0.029
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine
-
Eremothecium gossypii
2.5.1.9 0.04
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii AG33
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Eremothecium gossypii
-
-
-
2.5.1.9 Eremothecium gossypii AG33
-
-
-
2.5.1.9 Escherichia coli
-
strain ATCC 9637
-
2.5.1.9 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9
-
Escherichia coli
2.5.1.9
-
Saccharomyces cerevisiae
2.5.1.9
-
Eremothecium gossypii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Escherichia coli
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Saccharomyces cerevisiae
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Eremothecium gossypii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.9 mycelium
-
Eremothecium gossypii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.9 additional information
-
-
Saccharomyces cerevisiae
2.5.1.9 additional information
-
-
Eremothecium gossypii
2.5.1.9 0.003
-
-
Escherichia coli
2.5.1.9 0.052
-
purified enzyme Saccharomyces cerevisiae
2.5.1.9 0.266
-
purified enzyme Eremothecium gossypii

Storage Stability

EC Number Storage Stability Organism
2.5.1.9 -20°C, completely purified enzyme, stable for several months Saccharomyces cerevisiae
2.5.1.9 frozen, partially purified in dissolved ammonium sulfate precipitation pellet, stable several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Eremothecium gossypii riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Eremothecium gossypii AG33 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 additional information
-
Escherichia coli ?
-
?
2.5.1.9 additional information overview Saccharomyces cerevisiae ?
-
?
2.5.1.9 additional information overview Eremothecium gossypii ?
-
?
2.5.1.9 additional information overview Eremothecium gossypii AG33 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.9 37
-
assay at Escherichia coli
2.5.1.9 37
-
assay at Saccharomyces cerevisiae
2.5.1.9 37
-
assay at Eremothecium gossypii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.9
-
2 cold lability, inclusion of 0.01 M sodium sulfite in the solution used in purification prevents inactivation Saccharomyces cerevisiae
2.5.1.9 92
-
1 min, complete loss of activity Eremothecium gossypii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 6.9
-
-
Eremothecium gossypii
2.5.1.9 7
-
-
Escherichia coli
2.5.1.9 7
-
-
Saccharomyces cerevisiae