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Literature summary extracted from

  • Della Ragione, F.; Carteni-Farina, M.; Gragnaniello, V.; Schettino, M.I.; Zappia, V.
    Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta (1986), J. Biol. Chem., 261, 12324-12329.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.28 dithiothreitol required Homo sapiens
2.4.2.28 SH-group reducing agents requirement Homo sapiens

General Stability

EC Number General Stability Organism
2.4.2.28 rapid inactivation in absence of reducing agents: 50% inactivation within 24 h at both 4°C and -20°C Homo sapiens
2.4.2.28 resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 DTNB
-
 Homo sapiens
2.4.2.28 iodoacetamide no inhibition Homo sapiens
2.4.2.28 iodoacetate no inhibition Homo sapiens
2.4.2.28 N-ethylmaleimide 5'-methylthioadenosine partly protects Homo sapiens
2.4.2.28 p-chloromercuribenzoic acid
-
 Homo sapiens
2.4.2.28 SH-group blocking compounds
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.005
-
 5'-methylthioadenosine
-
 Homo sapiens
2.4.2.28 0.0055
-
 5'-methylthioadenosine
-
 Homo sapiens
2.4.2.28 0.008
-
 5-methylthioribose 1-phosphate
-
 Homo sapiens
2.4.2.28 0.023
-
 adenine
-
 Homo sapiens
2.4.2.28 0.32
-
 phosphate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 32500
-
 3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol Homo sapiens
2.4.2.28 98000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.28 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 10.2
-
 purified enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 -20°C, 5 mM DTT, 50 mM potassium phosphate, pH 7.4, less than 10% loss of activity after 1 month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.28 trimer 3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 additional information
-
 resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate Homo sapiens
2.4.2.28 40 55 15 min, stable Homo sapiens
2.4.2.28 70
-
 15 min, complete loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 additional information
-
 pI: 5.5 Homo sapiens
2.4.2.28 7.1 7.6
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.28 6.2 8.6 50% of activity maximum at pH 6.2 and pH 8.6 Homo sapiens