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Literature summary extracted from

  • Zappia, V.; Oliva, A.; Cacciapuoti, G.; Galletti, P.; Mignucci, G.; Carteni-Farina, M.
    Substrate specificity of 5'-methylthioadenosine phosphorylase from human prostate (1978), Biochem. J., 175, 1043-1050.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.28 dithiothreitol required Homo sapiens
2.4.2.28 dithiothreitol other thiols are less effective Homo sapiens
2.4.2.28 SH-group reducing agents requirement Homo sapiens

General Stability

EC Number General Stability Organism
2.4.2.28 Stable to freeze-thawing Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 5'-dimethylthioadenosine sulfonium salt, noncompetitive Homo sapiens
2.4.2.28 5'-methylthiotubercidin competitive Homo sapiens
2.4.2.28 iodoacetamide reversal by dithiothreitol Homo sapiens
2.4.2.28 iodoacetate
-
 Homo sapiens
2.4.2.28 p-chloromercuribenzoic acid strong, partially reversed by dithiothreitol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.025
-
 5'-methylthioadenosine
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.2.28 O2 partially inactivates Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate 3 binding sites between substrate and enzyme Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.28 prostate gland
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 0.131
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-ethylthioadenosine + phosphate 60% of the activity with 5'-methylthioadenosine Homo sapiens adenine + 5-ethylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylselenoadenosine + phosphate 95% of the activity with 5'-methylthioadenosine Homo sapiens adenine + 5-methylseleno-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate completely dependent on phosphate Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioinosine + phosphate 8.9% of the reaction with 5'-methylthioadenosine Homo sapiens hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 additional information substrate specificity Homo sapiens ?
-
 ?
2.4.2.28 additional information replacement of the 6-amino group by an OH-group and of N-7 by a methinic radical results in almost complete loss of activity Homo sapiens ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 65
-
 rapid inactivation Homo sapiens