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Literature summary extracted from

  • Zappia, V.; Carteni-Farina, M.; Romeo, G.; De Rosa, M.; Gambacorta, A.
    Purification and properties of 5'-methyladenosine phosphorylase from Caldariella acidophila (1983), Methods Enzymol., 94, 355-361.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.095
-
 5'-methylthioadenosine
-
 Caldariella acidophila
2.4.2.28 6.1
-
 phosphate
-
 Caldariella acidophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate Caldariella acidophila an antiproliferative effect on stimulated human lymphocytes and virally transformed mouse fibroblasts adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate Caldariella acidophila involved in salvage of adenine and methionine from 5'-methylthioadenosine adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate Caldariella acidophila physiological significance of 5'-methylthioadenosine cleavage is probably related to removal of the thioether which in turn exerts a significant inhibition on methyl transfer reactions adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Caldariella acidophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28
-
 Caldariella acidophila

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate sequential mechanism Caldariella acidophila
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 2.46
-
 purified enzyme Caldariella acidophila

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 -20°C, stable for several months Caldariella acidophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-isobutylthioadenosine + phosphate 97% of the reaction with 5'-methylthioadenosine Caldariella acidophila adenine + 5-isobutylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-isobutylthioinosine + phosphate 8.1% of the reaction with 5'-methylthioadenosine Caldariella acidophila hypoxanthine + 5-isobutylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate an antiproliferative effect on stimulated human lymphocytes and virally transformed mouse fibroblasts Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate involved in salvage of adenine and methionine from 5'-methylthioadenosine Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioadenosine + phosphate physiological significance of 5'-methylthioadenosine cleavage is probably related to removal of the thioether which in turn exerts a significant inhibition on methyl transfer reactions Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-methylthioinosine + phosphate
-
 Caldariella acidophila hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-n-butylthioadenosine + phosphate 93.3% of the reaction with 5'-methylthioadenosine Caldariella acidophila adenine + 5-n-butylthio-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 70
-
 assay at Caldariella acidophila
2.4.2.28 95
-
-
 Caldariella acidophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.28 86 95 86°C: 50% of activity maximum, 95°C: activity maximum, no activity at 40°C Caldariella acidophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 100
-
 1 h, stable Caldariella acidophila