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Literature summary extracted from

  • Ryu, K.Y.; Baik, Y.J.; Yang, C.H.
    Purification and characterization of farnesyl protein transferase from bovine testis (1995), J. Biochem. Mol. Biol., 28, 197-203.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.58 additional information n-octyl beta-D-glycopyranoside stimulates enzyme activity even at concentrations of 0.5% Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.58 EDTA
-
 Bos taurus
2.5.1.58 Lys-Lys-Cys-Val-Ile-Met 50% inhibition at 0.0025 mM Bos taurus
2.5.1.58 sodium deoxycholate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.58 additional information
-
 additional information
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.58 Mg2+ required Bos taurus
2.5.1.58 Zn2+ required Bos taurus
2.5.1.58 Zn2+ zinc metalloenzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.58 100000
-
 gel filtration Bos taurus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.58 additional information n-octyl beta-D-glycopyranoside stimulates even at concentrations of 0.5% Bos taurus
2.5.1.58 additional information EDTA-treatment decreases activity Bos taurus
2.5.1.58 Triton X-100 and Zwittergent 3-14, no effect on the activity of the enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.58 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.58
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.58 testis
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.58 0.0207
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine posttranslational lipid modification in which a 15-carbon farnesyl isoprenoid is linked via a thioether bond to specific cysteine residues of proteins, the reactive cysteine is located in the C-terminal Ca1a2X motif in which C is the modified cysteine, a1 and a2 are often an aliphatic residue, and X is Ser, Met, Ala or Gln Bos taurus diphosphate + S-farnesyl protein
-
 ?
2.5.1.58 farnesyl diphosphate + protein-cysteine substrate: Ras-CVIM and GST-PEP Bos taurus diphosphate + S-farnesyl protein
-
 ?
2.5.1.58 farnesyl diphosphate + Ras-Cys-Val-Ile-Met
-
 Bos taurus diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.58 heterodimer
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.58 50
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.58 7.2
-
-
 Bos taurus