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Literature summary extracted from

  • Chen, W.J.; Moomaw, J.F.; Overton, L.; Kost, T.A.; Casey, P.J.
    High level expression of mammalian protein farnesyltransferase in a baculovirus system (1993), J. Biol. Chem., 286, 9675-9680.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.58 additional information infection of Sf9 cells with the recombinant baculovirus containing DNA sequences encoding both enzyme activity results in a dramatic enhancement of enzyme activity Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.58 coexpression of alpha and beta subunits of enzyme by Sf9 cells infected with a recombinant baculovirus Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.58 Cys-Val-Phe-Met
-
 Rattus norvegicus
2.5.1.58 additional information hexapeptide containing a switch of leucine for methionine at the COOH terminus or a switch of cysteine for serine at the prenylation site is essentially inactive in competing for enzyme activity Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.58 Co2+
-
 Rattus norvegicus
2.5.1.58 additional information not: Ca2+ Rattus norvegicus
2.5.1.58 additional information Co2+ Rattus norvegicus
2.5.1.58 Zn2+ zinc metalloenzyme Rattus norvegicus
2.5.1.58 Zn2+ enzyme contains a stoichiometric amount of zinc Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.58 46000
-
 alpha,beta, 1 * 48000 + 1 * 46000 Rattus norvegicus
2.5.1.58 48000
-
 alpha,beta, 1 * 48000 + 1 * 46000 Rattus norvegicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.58 additional information EDTA-treatment decreases activity Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.58 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.58 from recombinant baculovirus-infected Sf9 cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.58 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.58 0.0085
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F Rattus norvegicus diphosphate + S-farnesyl protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.58 heterodimer SDS-PAGE Rattus norvegicus
2.5.1.58 heterodimer alpha,beta, 1 * 48000 + 1 * 46000 Rattus norvegicus