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Literature summary extracted from

  • Neufeld, E.F.; Feingold, D.S.; Ilves, S.M.; Kessler, G.; Hassid, W.Z.
    Phosphorylation of D-galacturonic acid by extracts from germinating seeds of Phaseolus aureus (1961), J. Biol. Chem., 236, 3102-3105.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.44 mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity Vigna radiata var. radiata
2.7.1.44 mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity Pisum sativum
2.7.1.44 mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity Petroselinum crispum
2.7.1.44 mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity Raphanus sativus
2.7.1.44 mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity Fagopyrum esculentum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.44 Co2+ divalent metal ion is required, lower activity than Mg2+ or Mn2+ Vigna radiata var. radiata
2.7.1.44 Co2+ divalent metal ion is required, lower activity than Mg2+ or Mn2+ Pisum sativum
2.7.1.44 Co2+ divalent metal ion is required, lower activity than Mg2+ or Mn2+ Petroselinum crispum
2.7.1.44 Co2+ divalent metal ion is required, lower activity than Mg2+ or Mn2+ Raphanus sativus
2.7.1.44 Co2+ divalent metal ion is required, lower activity than Mg2+ or Mn2+ Fagopyrum esculentum
2.7.1.44 Mg2+ divalent metal ion is required, Zn2+ or Ni2+ are ineffective Vigna radiata var. radiata
2.7.1.44 Mg2+ divalent metal ion is required, Zn2+ or Ni2+ are ineffective Pisum sativum
2.7.1.44 Mg2+ divalent metal ion is required, Zn2+ or Ni2+ are ineffective Petroselinum crispum
2.7.1.44 Mg2+ divalent metal ion is required, Zn2+ or Ni2+ are ineffective Raphanus sativus
2.7.1.44 Mg2+ divalent metal ion is required, Zn2+ or Ni2+ are ineffective Fagopyrum esculentum
2.7.1.44 Mn2+ divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective Vigna radiata var. radiata
2.7.1.44 Mn2+ divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective Pisum sativum
2.7.1.44 Mn2+ divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective Petroselinum crispum
2.7.1.44 Mn2+ divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective Raphanus sativus
2.7.1.44 Mn2+ divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective Fagopyrum esculentum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.44 ATP + D-galacturonate Vigna radiata var. radiata involved in formation of pectin ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate Pisum sativum involved in formation of pectin ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate Petroselinum crispum involved in formation of pectin ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate Raphanus sativus involved in formation of pectin ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate Fagopyrum esculentum involved in formation of pectin ADP + 1-phospho-alpha-D-galacturonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.44 Fagopyrum esculentum
-
 buckwheat
-
2.7.1.44 Petroselinum crispum
-
 parsley
-
2.7.1.44 Pisum sativum
-
 pea
-
2.7.1.44 Raphanus sativus
-
 radish
-
2.7.1.44 Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.44 partial Vigna radiata var. radiata
2.7.1.44 partial Pisum sativum
2.7.1.44 partial Petroselinum crispum
2.7.1.44 partial Raphanus sativus
2.7.1.44 partial Fagopyrum esculentum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.44 leaf
-
 Petroselinum crispum
-
2.7.1.44 root
-
 Raphanus sativus
-
2.7.1.44 seed germinating Vigna radiata var. radiata
-
2.7.1.44 seed germinating Pisum sativum
-
2.7.1.44 seed germinating Fagopyrum esculentum
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.44 4°C, 0.05 M mercaptoethanol, stable up to 10 days Vigna radiata var. radiata
2.7.1.44 4°C, 0.05 M mercaptoethanol, stable up to 10 days Pisum sativum
2.7.1.44 4°C, 0.05 M mercaptoethanol, stable up to 10 days Petroselinum crispum
2.7.1.44 4°C, 0.05 M mercaptoethanol, stable up to 10 days Raphanus sativus
2.7.1.44 4°C, 0.05 M mercaptoethanol, stable up to 10 days Fagopyrum esculentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.44 ATP + D-galacturonate
-
 Vigna radiata var. radiata ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate
-
 Pisum sativum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate
-
 Petroselinum crispum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate
-
 Raphanus sativus ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate
-
 Fagopyrum esculentum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate involved in formation of pectin Vigna radiata var. radiata ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate involved in formation of pectin Pisum sativum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate involved in formation of pectin Petroselinum crispum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate involved in formation of pectin Raphanus sativus ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 ATP + D-galacturonate involved in formation of pectin Fagopyrum esculentum ADP + 1-phospho-alpha-D-galacturonate
-
 ?
2.7.1.44 additional information D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides Vigna radiata var. radiata ?
-
 ?
2.7.1.44 additional information D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides Pisum sativum ?
-
 ?
2.7.1.44 additional information D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides Petroselinum crispum ?
-
 ?
2.7.1.44 additional information D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides Raphanus sativus ?
-
 ?
2.7.1.44 additional information D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides Fagopyrum esculentum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.44 37
-
 assay at Vigna radiata var. radiata
2.7.1.44 37
-
 assay at Pisum sativum
2.7.1.44 37
-
 assay at Petroselinum crispum
2.7.1.44 37
-
 assay at Raphanus sativus
2.7.1.44 37
-
 assay at Fagopyrum esculentum