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Literature summary extracted from

  • Rees, D.A.
    Enzymatic desulphation of porphyran (1961), Biochem. J., 80, 449-453.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.5 Borate 60% activation at pH 7.6 Porphyra sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.5 Al3+
-
 Porphyra sp.
2.5.1.5 Ca2+
-
 Porphyra sp.
2.5.1.5 Cd2+
-
 Porphyra sp.
2.5.1.5 Cu2+
-
 Porphyra sp.
2.5.1.5 EDTA
-
 Porphyra sp.
2.5.1.5 KCN
-
 Porphyra sp.
2.5.1.5 NH4F
-
 Porphyra sp.
2.5.1.5 Ni2+
-
 Porphyra sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.5 additional information activity is dependent on the presence of a bi- or tervalent cation which is not Mg2+ Porphyra sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.5 porphyran Porphyra sp. eliminates sulfate from the D-galactose 6-sulfate residues of porphyran producing 3,6-anhydrogalactose residues sulfate + porphyran containing 3,6-anhydrogalactose residues
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.5 Porphyra sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.5 partial Porphyra sp.

Storage Stability

EC Number Storage Stability Organism
2.5.1.5 4°C, gradual loss of activity in solution Porphyra sp.
2.5.1.5 room temperature, stable for at least several months as a freeze-dried powder Porphyra sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.5 porphyran eliminates sulfate from the D-galactose 6-sulfate residues of porphyran producing 3,6-anhydrogalactose residues Porphyra sp. sulfate + porphyran containing 3,6-anhydrogalactose residues
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.5 7.6 7.8 sodium tetraborate/HCl buffer Porphyra sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.5 6.8 9.2 pH 6.8: about 45% of activity maximum, pH 9.2: about 35% of activity maximum Porphyra sp.