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Literature summary extracted from

  • Lee, S.L.; Floss, H.G.; Heinstein, P.
    Purification and properties of dimethylallylpyrophosphate:tryptophan dimethylallyl transferase, the first enzyme of ergot alkaloid biosynthesis in Claviceps. sp. SD 58 (1976), Arch. Biochem. Biophys., 177, 84-94.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.34 repeated thawing and freezing greatly accelerates denaturation Claviceps sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.34 Ca2+ no inhibition, even at 5 mM Claviceps sp.
2.5.1.34 Co3+
-
 Claviceps sp.
2.5.1.34 diphosphate noncompetitive to dimethylallyl diphosphate Claviceps sp.
2.5.1.34 Hg2+
-
 Claviceps sp.
2.5.1.34 L-4-(gamma,gamma-dimethylallyl)tryptophan product inhibition, competitive to dimethylallyl diphosphate, noncompetitive to L-tryptophan Claviceps sp.
2.5.1.34 Mg2+ inhibition above 5 mM Claviceps sp.
2.5.1.34 Mn2+
-
 Claviceps sp.
2.5.1.34 Zn2+
-
 Claviceps sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.067
-
 L-tryptophan 30°C Claviceps sp.
2.5.1.34 0.2
-
 dimethylallyl diphosphate 30°C Claviceps sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 Ca2+ partial activation Claviceps sp.
2.5.1.34 Cu2+ slight stimulation Claviceps sp.
2.5.1.34 Fe2+ activates Claviceps sp.
2.5.1.34 Li+ slight stimulation Claviceps sp.
2.5.1.34 Mg2+ activates Claviceps sp.
2.5.1.34 Mg2+ optimal concentration: 3-5 mM Claviceps sp.
2.5.1.34 Na+ slight stimulation Claviceps sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 70000
-
 1 * 70000, SDS-PAGE Claviceps sp.
2.5.1.34 210000
-
 gel filtration Claviceps sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan Claviceps sp. first pathway-specific enzyme of ergot alkaloid biosynthesis diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan Claviceps sp. SD58 first pathway-specific enzyme of ergot alkaloid biosynthesis diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Claviceps sp.
-
 SD58
-
2.5.1.34 Claviceps sp. SD58
-
 SD58
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34
-
 Claviceps sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.34 dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan sequential mechanism Claviceps sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.34 mycelium
-
 Claviceps sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.34 0.309
-
-
 Claviceps sp.

Storage Stability

EC Number Storage Stability Organism
2.5.1.34 -20°C, 10 mM Tris-HCl, pH 8.0, 20 mM CaCl2, 20 mM 2-mercaptoethanol, 10% glycerol, 2 mg/ml enzyme concentration, stable for 1 week Claviceps sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 5-methyltryptophan 7.4% of the activity with L-tryptophan Claviceps sp. diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 5-methyltryptophan 7.4% of the activity with L-tryptophan Claviceps sp. SD58 diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 6-methyltryptophan 9.8% of the activity with tryptophan Claviceps sp. diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 6-methyltryptophan 9.8% of the activity with tryptophan Claviceps sp. SD58 diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 7-methyltryptophan 40.5% of the activity with tryptophan Claviceps sp. diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 7-methyltryptophan 40.5% of the activity with tryptophan Claviceps sp. SD58 diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + bishomotryptophan 2% of the activity with tryptophan Claviceps sp. diphosphate + bis[4-(3-methylbut-2-enyl)]-L-homotryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan first pathway-specific enzyme of ergot alkaloid biosynthesis Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan first pathway-specific enzyme of ergot alkaloid biosynthesis Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 monomer 1 * 70000, SDS-PAGE Claviceps sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.34 50
-
 1 h, complete loss of activity Claviceps sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7 7.5
-
 Claviceps sp.