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Literature summary extracted from

  • Levin, D.H.; Racker, E.
    2-Keto-3-deoxy-8-phosphooctonic acid synthetase (1966), Methods Enzymol., 8, 216-221.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.55 dialysis against 0.01 M Tris-HCl, pH 7.5, containing 0.01 M thioglycerol for 4 h, less than 20% loss of activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 Co2+ 1 mM, 22% inhibition Pseudomonas aeruginosa
2.5.1.55 Cu2+ 1 mM, 80% inhibition Pseudomonas aeruginosa
2.5.1.55 EDTA
-
 Pseudomonas aeruginosa
2.5.1.55 Fe2+ 1 mM, 30% inhibition Pseudomonas aeruginosa
2.5.1.55 Mn2+ 1 mM, 40% inhibition Pseudomonas aeruginosa
2.5.1.55 phosphate
-
 Pseudomonas aeruginosa
2.5.1.55 Zn2+ 1 mM, 34% inhibition Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Pseudomonas aeruginosa
-
 pyocyaneus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.55 0.038
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate absolute specificity for phosphoenolpyruvate and D-arabinose-5-phosphate Pseudomonas aeruginosa 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
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