Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Warrilow, A.G.S.; Hawkesford, M.J.
    Cysteine synthase (O-acetylserine (thiol) lyase) substrate specificities classify the mitochondrial isoform as a cyanoalanine synthase (2000), J. Exp. Bot., 51, 985-993.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 chloroalanine substrate inhibition Spinacia oleracea
2.5.1.47 L-cysteine substrate inhibition Spinacia oleracea
2.5.1.47 O-acetylserine substrate inhibition Spinacia oleracea
2.5.1.47 Sulfide substrate inhibition Spinacia oleracea
4.4.1.9 3-chloro-L-alanine substrate inhibition Spinacia oleracea
4.4.1.9 L-cysteine substrate inhibition Spinacia oleracea
4.4.1.9 O-acetyl-L-serine substrate inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.108
-
 cysteine pH 8.0, 26°C, cysteine synthase A Spinacia oleracea
2.5.1.47 0.201
-
 cysteine pH 8.0, 26°C, cysteine synthase B Spinacia oleracea
2.5.1.47 0.785
-
 chloroalanine pH 8.0, 26°C, cysteine synthase B Spinacia oleracea
2.5.1.47 1.03
-
 O-acetylserine pH 8.0, 26°C, cysteine synthase B Spinacia oleracea
2.5.1.47 1.053
-
 chloroalanine pH 8.0, 26°C, cysteine synthase A Spinacia oleracea
2.5.1.47 1.355
-
 O-acetylserine pH 8.0, 26°C, cysteine synthase A Spinacia oleracea
4.4.1.9 0.41
-
 L-cysteine 26°C, pH 9.8 Spinacia oleracea
4.4.1.9 0.6
-
 3-chloro-L-alanine 26°C, pH 9.8 Spinacia oleracea
4.4.1.9 9.73
-
 O-acetyl-L-serine 26°C, pH 9.8 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.9 33500
-
 2 * 33500, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.9 L-cysteine + HCN Spinacia oleracea
-
 sulfide + L-3-cyanoalanine
-
 ?
4.4.1.9 O-acetyl-L-serine + sulfide Spinacia oleracea
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Spinacia oleracea
-
 L. cv. Medina
-
4.4.1.9 Spinacia oleracea
-
 L. cv. Medina
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 isoenzyme A and B Spinacia oleracea
4.4.1.9
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Spinacia oleracea
-
4.4.1.9 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 chloroalanine + sulfide 3-6% of the activity of the cysteine synthase reaction Spinacia oleracea cysteine + chloride
-
 r
2.5.1.47 cysteine + CN-
-
 Spinacia oleracea cyanoalanine + H2S H2S i.e. bisulfide ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Spinacia oleracea L-Cys + acetate
-
 r
4.4.1.9 chloroalanine + sulfide
-
 Spinacia oleracea L-cysteine + Cl-
-
 ?
4.4.1.9 L-cysteine + HCN
-
 Spinacia oleracea sulfide + L-3-cyanoalanine
-
 ?
4.4.1.9 L-cysteine + HCN 73% of activity with chloroanaline Spinacia oleracea sulfide + L-3-cyanoalanine
-
 ?
4.4.1.9 O-acetyl-L-serine + sulfide
-
 Spinacia oleracea L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + sulfide 2.35% of activity with chloroanaline Spinacia oleracea L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.9 dimer 2 * 33500, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 9 11.5
-
 Spinacia oleracea
4.4.1.9 10
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.9 10 11.5 sharp decrease in activity below pH 10.0, 15% of maximal activity at pH 11.5 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.9 pyridoxal 5'-phosphate
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.47 0.8
-
 chloroalanine pH 9.8, 26 C, cysteine synthase B Spinacia oleracea
2.5.1.47 1.135
-
 chloroalanine pH 9.8, 26 C, cysteine synthase A Spinacia oleracea
2.5.1.47 2.074
-
 L-cysteine pH 8.0, 26 C, cysteine synthase A Spinacia oleracea
2.5.1.47 2.113
-
 cysteine pH 8.0, 26 C, cysteine synthase B Spinacia oleracea
2.5.1.47 5.546
-
 chloroalanine pH 8.0, 26 C, cysteine synthase B Spinacia oleracea
2.5.1.47 19.56
-
 O-acetylserine pH 8.0, 26 C, cysteine synthase B Spinacia oleracea
2.5.1.47 32.25
-
 O-acetylserine pH 8.0, 26 C, cysteine synthase A Spinacia oleracea
2.5.1.47 70.92
-
 chloroalanine pH 8.0, 26 C, cysteine synthase A Spinacia oleracea
4.4.1.9 5.22
-
 L-cysteine 26°C, pH 9.8 Spinacia oleracea
4.4.1.9 6.9
-
 3-chloro-L-alanine 26°C, pH 9.8 Spinacia oleracea
4.4.1.9 38.1
-
 O-acetyl-L-serine 26°C, pH 9.8 Spinacia oleracea