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Literature summary extracted from

  • Droux, M.; Martin, J.; Sajus, P.; Douce, R.
    Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts (1992), Arch. Biochem. Biophys., 295, 379-390.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.47 chloroplast enzyme very unstable Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.25
-
 H2S pH 7.5, 25°C, chloroplast enzyme Spinacia oleracea
2.5.1.47 1.3
-
 O-acetyl-L-Ser pH 7.5, 25°C, chloroplast enzyme Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 chloroplast different from cytosolic and mitochondrial enzymes, as revealed by polyclonal antibody studies, 3-5% of total activity Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 35000
-
 2 * 35000, SDS-PAGE Spinacia oleracea
2.5.1.47 68000
-
 chloroplast enzyme, gel filtration Spinacia oleracea
2.5.1.47 310000
-
 cysteine synthetase complex, gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 96
-
 chloroplast enzyme Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine
-
 Spinacia oleracea L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 35000, SDS-PAGE Spinacia oleracea
2.5.1.47 More amino acid composition Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 50
-
 chloroplast enzyme Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate 1.1 mol per mol subunit Spinacia oleracea