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Literature summary extracted from

  • Yamaguchi, T.; Masada, M.
    Comparative studies on cysteine synthase isozymes from spinach leaves (1995), Biochim. Biophys. Acta, 1251, 91-98.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 hydroxylamine complete inhibition at 10 mM, isoenzymes 1 and 2, 90% inhibition at 10 mM, isoenzyme 3 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.55
-
 H2S pH 8.0, 25°C, isoenzyme 1 Spinacia oleracea
2.5.1.47 1.25
-
 H2S pH 8.0, 25°C, isoenzyme 2 Spinacia oleracea
2.5.1.47 2.5
-
 H2S pH 8.0, 25°C, isoenzyme 3 Spinacia oleracea
2.5.1.47 3.57
-
 O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 1 Spinacia oleracea
2.5.1.47 5.26
-
 O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 3 Spinacia oleracea
2.5.1.47 5.56
-
 O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 2 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 34000
-
 2 * 34000, SDS-PAGE Spinacia oleracea
2.5.1.47 63000
-
 all isoenzymes, gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Spinacia oleracea
-
 three isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 400
-
 isoenzyme 1 Spinacia oleracea
2.5.1.47 1400
-
 isoenzyme 2 Spinacia oleracea
2.5.1.47 1431
-
 isoenzyme 3 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine
-
 Spinacia oleracea L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 34000, SDS-PAGE Spinacia oleracea
2.5.1.47 More amino acid composition Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 50
-
 2 min, all isoenzymes stable Spinacia oleracea
2.5.1.47 65
-
 2 min, isoenzyme 1 loses 50% loss of activity, isoenzymes 2 loses 25% of its activity, no loss of activity, isoenzyme 3 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8
-
 broad optimum around Spinacia oleracea