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Literature summary extracted from

  • Rosichan, J.L.; Blake, N.; Stallard, R.; Owais, W.M.; Kleinhofs, A.; Nilan, R.A.
    O-acetylserine (thiol)-lyase from barley converts sodium azide to a mutagenic metabolite (1983), Biochim. Biophys. Acta, 748, 367-373.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 NaN3
-
 Hordeum vulgare
2.5.1.47 O-acetylserine at 150 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 2.3
-
 NaN3 pH 7.6, 25°C Hordeum vulgare
2.5.1.47 8.2
-
 O-acetyl-L-Ser pH 7.6, 25°C Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 5500
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 NaN3 + O-acetyl-Ser
-
 Hordeum vulgare beta-azidoalanine + sodium acetate mutagenic in Salmonella typhimurium ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 50
-
 10 min, no loss of activity Hordeum vulgare
2.5.1.47 60
-
 10 min, 85% loss of activity Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8
-
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.47 2.3
-
 NaN3 pH 7.4, 25 C Hordeum vulgare