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Literature summary extracted from

  • Diessner, W.; Schmidt, A.
    Isoenzymes of cysteine synthase in the cyanobacterium Synechococcus 6301 (1981), Z. Pflanzenphysiol., 102, 57-68.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 L-cysteine
-
 Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.7
-
 O-acetyl-L-Ser pH 7.5, 37°C, isoenzyme 1 and 2 Synechococcus sp.
2.5.1.47 0.8
-
 H2S pH 7.5, 37°C, isoenzyme 1 and 2 Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 56000
-
 isoenzyme 1 and 2, gel filtration Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Synechococcus sp. last step of assimilatory sulfate reduction L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide Synechococcus sp. 6301 last step of assimilatory sulfate reduction L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Synechococcus sp.
-
 two isoenzymes
-
2.5.1.47 Synechococcus sp. 6301
-
 two isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Synechococcus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate model of reaction mechanism Synechococcus sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Synechococcus sp. L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Synechococcus sp. 6301 L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Synechococcus sp. L-cysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Synechococcus sp. 6301 L-cysteine + acetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 50
-
-
 Synechococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.47 45 65
-
 Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.5
-
-
 Synechococcus sp.