EC Number | General Stability | Organism |
---|---|---|
2.5.1.56 | a specific cleavage by endogenous proteases at Lys280 of the 40000 Da enzyme. Cleavage results in the formation of two inactive fragments of 33000 Da and 7000 Da. The fragmentation is associated with a significant change of the enzyme from a tetrameric to trimeric form, and alterations in both secondary and native quarternary structures | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 40538 | - |
4 * 40538, LC-MS analysis | Escherichia coli |
2.5.1.56 | 135000 | - |
recombinant protein containing a hexahistidine tag at the N-terminus, gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.56 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.56 | N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Escherichia coli | N-acetylneuraminate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.56 | tetramer | 4 * 40538, LC-MS analysis | Escherichia coli |