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Literature summary extracted from

  • Tabata, K.; Koizumi, S.; Endo, T.; Ozaki, A.
    Production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase (2002), Enzyme Microb. Technol., 30, 327-333.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.5.1.56 synthesis development of a novel microbial transformation for the synthesis of N-acetyl-D-neuraminic acid using bacterial cells expressing N-acetyl-D-glucosamine 2-epimerase and N-acetylneuraminate synthase Escherichia coli
5.1.3.8 synthesis production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase. Microbial production of N-acetylneuraminic acid is carried out using Escherichia coli overexpressing N-acylglucosamine 2-epimerase and NeuAc synthetase as enzyme sources. Phosphoenolpyruvate and ATP, required as a substrate or a cofactor of the enzymes, are supplied by the activities of Escherichia coli and Corynebacterium ammoniagenes cells. Starting with 800 mM GlcNAc and 360 mM glucose, NeuAc accumulates at 39.7 mM after 22 h Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.56
-
Escherichia coli
5.1.3.8 expression in Escherichia coli Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.8 4.76
-
N-acetyl-D-mannosamine
-
Synechocystis sp.
5.1.3.8 6.94
-
N-acetyl-D-glucosamine
-
Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Escherichia coli
-
-
-
5.1.3.8 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.8 recombinant enzyme Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Escherichia coli N-acetylneuraminate + phosphate
-
?
5.1.3.8 N-acetyl-D-mannosamine
-
Synechocystis sp. N-acetyl-D-glucosamine
-
r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.8 GlcNAc 2-epimerase
-
Synechocystis sp.
5.1.3.8 N-Acetylglucosamine 2-epimerase
-
Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.8 ATP essential for activity Synechocystis sp.