EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.56 | synthesis | development of a novel microbial transformation for the synthesis of N-acetyl-D-neuraminic acid using bacterial cells expressing N-acetyl-D-glucosamine 2-epimerase and N-acetylneuraminate synthase | Escherichia coli |
5.1.3.8 | synthesis | production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase. Microbial production of N-acetylneuraminic acid is carried out using Escherichia coli overexpressing N-acylglucosamine 2-epimerase and NeuAc synthetase as enzyme sources. Phosphoenolpyruvate and ATP, required as a substrate or a cofactor of the enzymes, are supplied by the activities of Escherichia coli and Corynebacterium ammoniagenes cells. Starting with 800 mM GlcNAc and 360 mM glucose, NeuAc accumulates at 39.7 mM after 22 h | Synechocystis sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.56 | - |
Escherichia coli |
5.1.3.8 | expression in Escherichia coli | Synechocystis sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.8 | 4.76 | - |
N-acetyl-D-mannosamine | - |
Synechocystis sp. | |
5.1.3.8 | 6.94 | - |
N-acetyl-D-glucosamine | - |
Synechocystis sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.56 | Escherichia coli | - |
- |
- |
5.1.3.8 | Synechocystis sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.8 | recombinant enzyme | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.56 | N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Escherichia coli | N-acetylneuraminate + phosphate | - |
? | |
5.1.3.8 | N-acetyl-D-mannosamine | - |
Synechocystis sp. | N-acetyl-D-glucosamine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.8 | GlcNAc 2-epimerase | - |
Synechocystis sp. |
5.1.3.8 | N-Acetylglucosamine 2-epimerase | - |
Synechocystis sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.1.3.8 | ATP | essential for activity | Synechocystis sp. |